SPARC (D10F10) Rabbit mAb #8725
- WB
- IHC
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 42 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:2400 |
Storage
For a carrier free (BSA and azide free) version of this product see product #70838.
Protocol
Specificity / Sensitivity
SPARC (D10F10) Rabbit mAb recognizes endogenous levels of total SPARC protein.
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val111 of human SPARC protein.
Background
SPARC (secreted protein acidic and rich in cysteine), also known as osteonectin and BM40, is a secreted matricellular glycoprotein that belongs to a group of functionally related glycoproteins that includes tenascins C and X, thrombospondins 1 and 2, and osteopontin (1). Members in this class of glycoproteins are involved in tissue renewal, tissue remodeling, and embryonic development and work by exerting counter-adhesive and antiproliferative effects that lead to changes in cell shape, disruption of cell adhesion, and inhibition of the cell cycle (2). SPARC is expressed at high levels in bone tissue but is widely distributed in many other tissues and cell types (3), and is known to be associated with tissues undergoing morphogenesis, angiogenesis, mineralization, and other pathological responses to injury and tumorigenesis (4,5). SPARC has also been linked with obesity and diabetes (6).
- Fukunaga-Kalabis, M. et al. (2008) Cancer Microenviron 1, 93-102.
- Yan, Q. and Sage, E.H. (1999) J Histochem Cytochem 47, 1495-506.
- Maillard, C. et al. (1992) Bone 13, 257-64.
- Sage, E.H. (1997) Nat Med 3, 144-6.
- Bradshaw, A.D. and Sage, E.H. (2001) J Clin Invest 107, 1049-54.
- Kos, K. and Wilding, J.P. (2010) Nat Rev Endocrinol 6, 225-35.
Limited Uses
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