Spermidine synthase (F5X6P) Rabbit mAb #43724

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Supporting Data

REACTIVITY	H M R
SENSITIVITY	Endogenous
MW (kDa)	33
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Spermidine synthase (F5X6P) Rabbit mAb recognizes endogenous levels of total spermidine synthase protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human spermidine synthase protein.

Background

Polyamines, including spermine, spermidine, and putrescine, are involved in many cellular processes, for example, cell proliferation, differentiation, apoptosis, the synthesis of proteins and nucleic acids, ion channel activity regulation, and the protection from oxidative injury (1). The cellular content of polyamines is tightly regulated; their biosynthesis is catalyzed by different enzymes including spermine synthase (SMS) and spermidine synthase (SRM), while the enzymes N1-acetylpolyamine oxidase (PAOX), spermidine/spermine N1-acetyltransferase 1 (SAT1), and spermine oxidase (SMOX) are involved in polyamine catabolism (1).

SRM catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (2). In the brain, SRM is predominantly localized to neurons and neuropil with low expression in glial cells (3,4).

Database Links

UniProt ID: P19623

Entrez-Gene Id: 6723

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For Research Use Only. Not For Use In Diagnostic Procedures.

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