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Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

SPHK1 Antibody detects endogenous levels of total SPHK1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human SPHK1. Antibodies are purified by peptide affinity chromatography.

Sphingosine kinases (SPHKs) catalyze the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Together with other sphingolipid metabolizing enzymes, SPHKs regulate the balance of the lipid mediators, ceramide, sphingosine, and S1P (1-4). Two distinct SPHK isoforms, SPHK1 and SPHK2, have been cloned and characterized (5,6). SPHK1 and SPHK2 are highly conserved and diversely expressed (7,8). The SPHKs are activated by G protein-coupled receptors, receptor tyrosine kinases, immunoglobulin receptors, cytokines, and other stimuli (9-12). The molecular mechanisms by which SPHK1 and SPHK2 are specifically regulated are complex and only partially understood.

1.  Hait, N.C. et al. (2006) Biochim Biophys Acta 1758, 2016-26.

2.  Xia, P. et al. (2000) Curr Biol 10, 1527-30.

3.  Hannun, Y.A. et al. (2001) Biochemistry 40, 4893-903.

4.  Futerman, A.H. and Riezman, H. (2005) Trends Cell Biol 15, 312-8.

5.  Kohama, T. et al. (1998) J Biol Chem 273, 23722-8.

6.  Liu, H. et al. (2000) J Biol Chem 275, 19513-20.

7.  Liu, H. et al. (2002) Prog Nucleic Acid Res Mol Biol 71, 493-511.

8.  Spiegel, S. and Milstien, S. (2003) Nat Rev Mol Cell Biol 4, 397-407.

9.  Alemany, R. et al. (2007) Naunyn Schmiedebergs Arch Pharmacol 374, 413-28.

10.  Saba, J.D. and Hla, T. (2004) Circ Res 94, 724-34.

11.  Anliker, B. and Chun, J. (2004) J Biol Chem 279, 20555-8.

12.  Wattenberg, B.W. et al. (2006) J Lipid Res 47, 1128-39.

Entrez-Gene Id 8877
Swiss-Prot Acc. Q9NYA1

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

SPHK1 Antibody