Render Target: STATIC
Render Timestamp: 2024-12-02T11:04:20.615Z
Commit: cd2fae6ca3f811b1ddb1df24ac291ed56d5d501b
XML generation date: 2024-09-30 01:57:45.756
Product last modified at: 2024-11-20T21:00:08.992Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

SPIN1 (E6R1Z) Rabbit mAb #89139

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 29
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SPIN1 (E6R1Z) Rabbit mAb recognizes endogenous levels of total SPIN1 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His33 of human SPIN1 protein.

    Background

    SPIN1, also known as Spindlin1, was first identified as a spindle binding protein during meiosis in mice (1). SPIN1 contains tandem tudor-like domains, which can recognize methylation of H3K4, H4K20, and H4R23 (2-4). SPIN1 localizes to rRNA genes to help drive their expression, and has also been shown to promote cellular proliferation through binding TCF4 to activate wnt signaling (2,5). This transcriptional activity is opposed by the SPIN1 docking protein SPINDOC, a repressor which causes SPIN1 to dissociate from chromatin (6). SPIN1 has shown to be overexpressed in ovarian cancers, and in other cell types SPIN1 overexpression causes cell cycle arrest and chromosome instability (7,8). SPIN1 has also been identified as a potential target in liposarcomas, where it contributes to RET signaling by controlling the expression of the activator GDNF (9).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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