Render Target: STATIC
Render Timestamp: 2024-12-06T12:16:31.001Z
Commit: 224419269841c11382c4555dbee545259bf6c379
XML generation date: 2024-09-30 01:57:05.549
Product last modified at: 2024-10-16T11:45:10.585Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Spred1 (D6D8B) Rabbit mAb #94063

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 50
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Spred1 (D6D8B) Rabbit mAb recognizes endogenous levels of total Spred1 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln281 of human Spred1 protein.

    Background

    Spred1 belongs to the Spred family functioning as sprouty-related suppressor of Ras signaling down stream of growth receptor signaling (1,2). The protein has a N-terminal EVH1 (Ena/VASP homology-1) domain, followed by a KBD (c-Kit- binding domain) and a C-terminal SPR (Sprouty) domain. The C-terminal SPR domain functions to translocate Spred1 to the membrane following growth factor stimulation (1,3). The N-terminal EVH1 domain interacts with neurofibromin to bring the later to the membrane proximal signaling complex for down regulation of Ras signaling (4,5). Mutations of Spred1 are the causes of Legius Syndrome (6,7). Spred1 expression inhibits tumor cell motility and metastasis, and down regulation has been found in hepatocarcinoma and myeloblastic leukemia (8-10).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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