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48046
SQSTM1/p62-like Receptor Antibody Sampler Kit

SQSTM1/p62-like Receptor Antibody Sampler Kit #48046

Western Blotting Image 1

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® SQSTM1/p62 siRNA I #6394 (+) or SignalSilence® SQSTM1/p62 siRNA II #6399 (+), using SQSTM1/p62 (D5E2) Rabbit mAb (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The SQSTM1/p62 (D5E2) Rabbit mAb confirms silencing of SQSTM1/p62 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.

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Western Blotting Image 2

Western blot analysis of extracts from various cell lines using Optineurin (D2L8S) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

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Western Blotting Image 3

Western blot analysis of extracts from various cell lines using NBR1 (D2E6) Rabbit mAb.

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Western Blotting Image 4

Western blot analysis of extracts from various cell lines using NDP52 (D1E4A) Rabbit mAb.

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Western Blotting Image 5

Western blot analysis of extracts from various cell lines using TAX1BP1 (D1D5) Rabbit mAb.

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Western Blotting Image 6

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

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Western Blotting Image 7

Western blot analysis of extracts from various cell lines using SQSTM1/p62 (D5E2) Rabbit mAb.

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Flow Cytometry Image 8

Flow cytometric analysis of MCF7 cells using NBR1 (D2E6) Rabbit mAb (blue) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).

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Western Blotting Image 9

Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected with a Myc/DDK-tagged cDNA expression construct encoding the full-length isoform 1 of human TAX1BP1 (hTAX1BP1-Myc/DDK, +), using TAX1BP1 (D1D5) Rabbit mAb.

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Western Blotting Image 10

Western blot analysis of extracts from SK-MEL-2 cells, untreated (-) or starved overnight in Earle's Balanced Salt Solution (EBSS) (+), using SQSTM1/p62 (D5E2) Rabbit mAb.

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IF-IC Image 11

Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with bafilomycin A (0.2 μM, 18 hr; right), using NBR1 (D2E6) Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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Western Blotting Image 12

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a tagged human SQSTM1/p62 construct (+), using SQSTM1/p62 (D5E2) Rabbit mAb.

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
SQSTM1/p62 (D5E2) Rabbit mAb 8025 20 µl
  • WB
  • IP
H Mk 62 Rabbit IgG
Optineurin (D2L8S) Rabbit mAb 58981 20 µl
  • WB
H 75 Rabbit IgG
NBR1 (D2E6) Rabbit mAb 9891 20 µl
  • WB
  • IP
  • IF
  • F
H M 120 Rabbit IgG
NDP52 (D1E4A) Rabbit mAb 60732 20 µl
  • WB
H 52, 60 Rabbit IgG
TAX1BP1 (D1D5) Rabbit mAb 5105 20 µl
  • WB
  • IP
H 92 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

The SQSTM1/p62-like Receptor Antibody Sampler Kit provides an economical means of detecting members of the SQSTM1/p62-like Receptor (SLR) family. The kit includes enough antibody to perform two western blot experiments with each primary antibody.

Each antibody in the SQSTM1/p62-like Receptor Antibody Sampler Kit detects endogenous levels of its target protein. Based on sequence alignment, TAX1BP (D1D5) Rabbit mAb is predicted to react with TXBP151-L and TXBP151-S isoforms.

Monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to Gly162 of human SQSTM1/p62, Lys601 of human NBR1, Leu410 of human Optineurin, Val135 of NDP52, and residues near the carboxy terminus of human TAX1BP1.

Autophagy is a catabolic process for the autophagosome-lysosomal degradation of bulk cytoplasmic contents (1,2). Selective autophagy targets the degradation of distinct sets of substrates and organelles and can occur through the utilization of a number of autophagy cargo receptors (3-5). Autophagy cargo receptors contain an LC3-interacting region (LIR) required for interaction with Atg8/LC3 family members targeted to the autophagosome. SQSTM1/p62-like receptors (SLRs) are a family of autophagy cargo receptors that contain domains for binding to ubiquitin. This family includes prototypical member SQSTM1/p62, NBR1, NDP52, Optineurin, and TAX1BP1. Targets of SLRs include ubiquitylated protein aggregates (aggrephagy), organelles such as mitochondria (mitoophagy) and peroxisomes (pexophagy), and intracellular bacteria (xenophagy).

Upon binding of cargo to these receptors, the complex is delivered to the autophagosome where both the cargo and receptor are degraded through the autophagic process. While some redundancy may exist among SLR family members, they can have unique activities. Many SLRs can have additional roles as scaffolding proteins for various signaling pathways. For example, SQSTM1/p62 interacts with KEAP1, a cytoplasmic inhibitor of NRF2, a key transcription factor involved in cellular responses to oxidative stress (6). Thus, accumulation of SQSTM1/p62 can lead to an increase in NRF2 activity.

  1. Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21.
  2. Codogno, P. and Meijer, A.J. (2005) Cell Death Differ 12 Suppl 2, 1509-18.
  3. Birgisdottir, Å.B. et al. (2013) J Cell Sci 126, 3237-47.
  4. Xu, Z. et al. (2015) Acta Biochim Biophys Sin (Shanghai) 47, 571-80.
  5. Mancias, J.D. and Kimmelman, A.C. (2016) J Mol Biol 428, 1659-80.
  6. Komatsu, M. et al. (2010) Nat Cell Biol 12, 213-23.
Entrez-Gene Id
4077 , 10241 , 10133 , 8878 , 8887
Swiss-Prot Acc.
Q14596 , Q13137 , Q96CV9 , Q13501 , Q86VP1
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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