Src Family Antibody Sampler Kit #9320
Product Information
Kit Usage Information
Protocols
- 2123: Western Blotting, Immunoprecipitation (Magnetic)
- 2796: Western Blotting, Immunoprecipitation (Magnetic), Immunohistochemistry (Paraffin)
- 2984: Western Blotting, Immunohistochemistry (Paraffin)
- 3201: Western Blotting, Immunoprecipitation (Magnetic)
- 4023: Western Blotting
- 4980: Western Blotting
- 7074: Western Blotting
Product Description
Specificity / Sensitivity
Each antibody in the Src Family Antibody Sampler Kit detects endogenous levels of its target protein and does not cross-react with other family members.
Source / Purification
Src (32G6) Monoclonal antibody is produced by immunizing animals with a GST-Src fusion protein containing amino acid residues 1-110 of human Src. The remaining monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Val399 of human Csk, Thr50 of human Lck, and the amino-terminal sequence of human Lyn. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser25 of human Fyn and the amino terminus of human Yes. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.
Background
The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).
The kinase Fyn plays a role in T-cell receptor signaling and also helps in memory formation and adhesion mediated signaling (3,4). Lymphocyte specific kinase Lck is essential in the differentiation and activation of T-cells (3). Hematopoietic Lyn kinase, also phosphorylated by Csk, is involved in the regulation of B-cell function, migration and development (5). The ubiquitously expressed kinase Yes acts downstream of several different cell surface receptors, including G-protein-coupled receptors, and is involved in the regulation of angiogenesis, the cell cycle and cell adhesion (6,7).
- Thomas, S.M. and Brugge, J.S. (1997) Annu Rev Cell Dev Biol 13, 513-609.
- Hunter, T. (1987) Cell 49, 1-4.
- Palacios, E.H. and Weiss, A. (2004) Oncogene 23, 7990-8000.
- Isosaka, T. et al. (2008) Eur. J. Neurosci. 28, 973-981.
- Seo, S. et al. (2001) J. Immunol. 166, 3710-3716.
- Summy, J.M. et al. (2003) J. Cell Sci. 116, 2585-2598.
- Werdich, X.Q. and Penn, J.S. (2005) Angiogenesis 8, 315-326.
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