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9320
Src Family Antibody Sampler Kit

Src Family Antibody Sampler Kit #9320

Western Blotting Image 1

Western blot analysis of cell extracts from various cell lines, using Src (32G6) Rabbit mAb.

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Western Blotting Image 2

Western blot analysis of extracts from various cell lines using Csk (C74C1) Rabbit mAb.

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Western Blotting Image 3

Western blot analysis of cell extracts from various cell lines, using Fyn Antibody.

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Western Blotting Image 4

Western blot analysis of extracts from Ramos and Jurkat cells using LcK (D88) XP® Rabbit mAb.

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Western Blotting Image 5

Western blot analysis of cell extracts from various cell types using Lyn (C13F9) Rabbit mAb.

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Western Blotting Image 6

Western blot analysis of extracts from various cell lines using Yes Antibody.

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Western Blotting Image 7

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

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IHC-P (paraffin) Image 8

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Lck (D88) XP® Rabbit mAb.

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IHC-P (paraffin) Image 9

Immunohistochemical analysis of paraffin-embedded non-hodgkins lymphoma using Lyn (C13F9) Rabbit mAb.

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IHC-P (paraffin) Image 10

Immunohistochemical analysis of paraffin-embedded human lymphoma using Lck (D88) XP® Rabbit mAb.

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IHC-P (paraffin) Image 11

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Lyn (C13F9) Rabbit mAb in the presence of control peptide (left) and antigen specific peptide (right).

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IHC-P (paraffin) Image 12

Immunohistochemical analysis of paraffin-embedded Colo205 (positive) (left) and HeLa (negative) (right) cell pellets using Lck (D88) XP® Rabbit mAb.

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IHC-P (paraffin) Image 13

Immunohistochemical analysis of paraffin-embedded human tonsil using Lck (D88) XP® Rabbit mAb.

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Src (32G6) Rabbit mAb 2123 20 µl
  • WB
  • IP
H M R Mk 60 Rabbit IgG
Csk (C74C1) Rabbit mAb 4980 20 µl
  • WB
H M R Mk Pg 50 Rabbit IgG
Fyn Antibody 4023 20 µl
  • WB
H M 59 Rabbit 
Lck (D88) XP® Rabbit mAb 2984 20 µl
  • WB
  • IHC
H 56 Rabbit IgG
Lyn (C13F9) Rabbit mAb 2796 20 µl
  • WB
  • IP
  • IHC
H M R Mk 56 Rabbit IgG
Yes Antibody 3201 20 µl
  • WB
  • IP
H Mk B 60 Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

The Src Family Antibody Sampler Kit provides an economical means of evaluating total levels of Src family member proteins. The kit contains enough primary and secondary antibody to perform two western blots with each antibody.

Each antibody in the Src Family Antibody Sampler Kit detects endogenous levels of its target protein and does not cross-react with other family members.

Src (32G6) Monoclonal antibody is produced by immunizing animals with a GST-Src fusion protein containing amino acid residues 1-110 of human Src. The remaining monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Val399 of human Csk, Thr50 of human Lck, and the amino-terminal sequence of human Lyn. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser25 of human Fyn and the amino terminus of human Yes. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).

The kinase Fyn plays a role in T-cell receptor signaling and also helps in memory formation and adhesion mediated signaling (3,4). Lymphocyte specific kinase Lck is essential in the differentiation and activation of T-cells (3). Hematopoietic Lyn kinase, also phosphorylated by Csk, is involved in the regulation of B-cell function, migration and development (5). The ubiquitously expressed kinase Yes acts downstream of several different cell surface receptors, including G-protein-coupled receptors, and is involved in the regulation of angiogenesis, the cell cycle and cell adhesion (6,7).

  1. Thomas, S.M. and Brugge, J.S. (1997) Annu Rev Cell Dev Biol 13, 513-609.
  2. Hunter, T. (1987) Cell 49, 1-4.
  3. Palacios, E.H. and Weiss, A. (2004) Oncogene 23, 7990-8000.
  4. Isosaka, T. et al. (2008) Eur. J. Neurosci. 28, 973-981.
  5. Seo, S. et al. (2001) J. Immunol. 166, 3710-3716.
  6. Summy, J.M. et al. (2003) J Cell Sci 116, 2585-98.
  7. Werdich, X.Q. and Penn, J.S. (2005) Angiogenesis 8, 315-326.
Entrez-Gene Id
1445 , 2534 , 3932 , 4067 , 6714 , 7525
Swiss-Prot Acc.
P41240 , P06241 , P06239 , P07948 , P12931 , P07947
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

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