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9320
Src Family Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Src Family Antibody Sampler Kit #9320

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Src Family Antibody Sampler Kit: Image 1

Western blot analysis of cell extracts from various cell lines, using Src (32G6) Rabbit mAb.

Src Family Antibody Sampler Kit: Image 2

Western blot analysis of extracts from various cell lines using Csk (C74C1) Rabbit mAb.

Src Family Antibody Sampler Kit: Image 3

Western blot analysis of cell extracts from various cell lines, using Fyn Antibody.

Src Family Antibody Sampler Kit: Image 4

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Lck (D88) XP® Rabbit mAb.

Src Family Antibody Sampler Kit: Image 5

Immunohistochemical analysis of paraffin-embedded non-hodgkins lymphoma using Lyn (C13F9) Rabbit mAb.

Src Family Antibody Sampler Kit: Image 6

Western blot analysis of extracts from various cell lines using Yes Antibody.

Src Family Antibody Sampler Kit: Image 7

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Src Family Antibody Sampler Kit: Image 8

Immunohistochemical analysis of paraffin-embedded human lymphoma using Lck (D88) XP® Rabbit mAb.

Src Family Antibody Sampler Kit: Image 9

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Lyn (C13F9) Rabbit mAb in the presence of control peptide (left) and antigen specific peptide (right).

Src Family Antibody Sampler Kit: Image 10

Immunohistochemical analysis of paraffin-embedded Colo205 (positive) (left) and HeLa (negative) (right) cell pellets using Lck (D88) XP® Rabbit mAb.

Src Family Antibody Sampler Kit: Image 11

Western blot analysis of cell extracts from various cell types using Lyn (C13F9) Rabbit mAb.

Src Family Antibody Sampler Kit: Image 12

Immunohistochemical analysis of paraffin-embedded human tonsil using Lck (D88) XP® Rabbit mAb.

Src Family Antibody Sampler Kit: Image 13

Western blot analysis of extracts from Ramos and Jurkat cells using LcK (D88) XP® Rabbit mAb.

To Purchase # 9320T
Product # Size Price
9320T
1 Kit  (6 x 20 µl) $ 445

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Src (32G6) Rabbit mAb 2123 20 µl
  • WB
  • IP
H M R Mk 60 Rabbit IgG
Csk (C74C1) Rabbit mAb 4980 20 µl
  • WB
H M R Mk Pg 50 Rabbit IgG
Fyn Antibody 4023 20 µl
  • WB
H M 59 Rabbit 
Lck (D88) XP® Rabbit mAb 2984 20 µl
  • WB
  • IHC
H 56 Rabbit IgG
Lyn (C13F9) Rabbit mAb 2796 20 µl
  • WB
  • IP
  • IHC
H M R Mk 56 Rabbit IgG
Yes Antibody 3201 20 µl
  • WB
  • IP
H Mk B 60 Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Product Description

The Src Family Antibody Sampler Kit provides an economical means of evaluating total levels of Src family member proteins. The kit contains enough primary and secondary antibody to perform two western blots with each antibody.

Specificity / Sensitivity

Each antibody in the Src Family Antibody Sampler Kit detects endogenous levels of its target protein and does not cross-react with other family members.

Source / Purification

Src (32G6) Monoclonal antibody is produced by immunizing animals with a GST-Src fusion protein containing amino acid residues 1-110 of human Src. The remaining monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Val399 of human Csk, Thr50 of human Lck, and the amino-terminal sequence of human Lyn. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser25 of human Fyn and the amino terminus of human Yes. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Background

The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).

The kinase Fyn plays a role in T-cell receptor signaling and also helps in memory formation and adhesion mediated signaling (3,4). Lymphocyte specific kinase Lck is essential in the differentiation and activation of T-cells (3). Hematopoietic Lyn kinase, also phosphorylated by Csk, is involved in the regulation of B-cell function, migration and development (5). The ubiquitously expressed kinase Yes acts downstream of several different cell surface receptors, including G-protein-coupled receptors, and is involved in the regulation of angiogenesis, the cell cycle and cell adhesion (6,7).

  1. Thomas, S.M. and Brugge, J.S. (1997) Annu Rev Cell Dev Biol 13, 513-609.
  2. Hunter, T. (1987) Cell 49, 1-4.
  3. Palacios, E.H. and Weiss, A. (2004) Oncogene 23, 7990-8000.
  4. Isosaka, T. et al. (2008) Eur. J. Neurosci. 28, 973-981.
  5. Seo, S. et al. (2001) J Immunol 166, 3710-6.
  6. Summy, J.M. et al. (2003) J Cell Sci 116, 2585-98.
  7. Werdich, X.Q. and Penn, J.S. (2005) Angiogenesis 8, 315-326.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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