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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

SRRM2 (Nuclear Speckle Marker) (F3J1Z) Rabbit mAb #19700

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  • WB
  • IP
  • IF

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 200-300
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Immunofluorescence (Immunocytochemistry) 1:2000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SRRM2 (Nuclear Speckle Marker) (F3J1Z) Rabbit mAb recognizes endogenous levels of total SRRM2 protein. This antibody detects a 42 kDa protein of unknown identity in some cell lines.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the central region of human SRRM2 protein.

    Background

    Nuclear speckles (NS) are distinct biomolecular condensates consisting of nuclear protein and RNA molecules. Although their exact biological function is not fully understood, NS are believed to function as hubs for mRNA splicing and potentially transcription (1). The splicing factors serine/arginine repetitive matrix protein 2 (SRRM2) and SON are both considered necessary for NS formation (2). Both SRRM2 and SON undergo phase separation and function as scaffolding proteins, recruiting additional splicing factors and promoting NS assembly (3). In Alzheimer’s disease (AD) and other tauopathies, SRRM2 can become mislocalized from NS to pathological tau aggregates in the cytoplasm, resulting in aberrant splicing (4,5). Loss-of-function (LoF) mutations in SRRM2 have also been associated with neurodevelopmental delay and an increased risk of developing papillary thyroid carcinoma (PTC) (6,7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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