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SRSF2 (F1P3N) Rabbit Monoclonal Antibody #50901

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  • WB

    Product Specifications

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 35
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SRSF2 (F1P3N) Rabbit Monoclonal Antibody recognizes endogenous levels of total SRSF2 protein. This antibody detects a 22 kDa protein and a 140 kDa protein of unknown identities in some cell lines.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser101 of human SRSF2 protein.

    Background

    Serine/arginine-rich splicing factor 2 (SRSF2/SFRS2), also known as splicing factor SC35, is a well-studied serine/arginine-rich splicing factor (SR) protein. SR proteins are crucial regulators in precursor RNA splicing, a process associated with maintaining genome stability, regulating mRNA export and stability, and translation (1,2). SRSF2 and other SR proteins harbor an N-terminal RNA recognition motif (RRM) that is involved in sequence-specific RNA binding and a C-terminal arginine/serine-rich (RS) domain that facilitates interactions with other splicing proteins (2-5). Similar to most other SR proteins, SRSF2 is essential for normal development as its germline deletion is embryonically lethal in mice (6,7). Moreover, mutations in the SRSF2 gene are often found in myelodysplasia, with the most common hotspot mutation, a substitution of proline for histidine at position 95, causing altered recognition of exonic splicing enhancers and aberrantly spliced transcripts (8-10). In myeloid malignancies, SRSF2 mutations have been linked to abnormal differentiation of hematopoietic stem and progenitor cells (11).

    Alternate Names

    PR264; Protein PR264; SC-35; SC35; serine and arginine rich splicing factor 2; Serine/arginine-rich splicing factor 2; SFRS2; SFRS2A; Splicing component, 35 kDa; Splicing factor SC35; Splicing factor, arginine/serine-rich 2; SR splicing factor 2; SRp30b; SRSF2

    For Research Use Only. Not for Use in Diagnostic Procedures.
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