Render Target: STATIC
Render Timestamp: 2024-12-10T10:57:48.803Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-09-30 01:59:35.113
Product last modified at: 2024-11-20T13:15:11.481Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

SRSF7 (F9G2P) Rabbit mAb #82637

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 35
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:10 - 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SRSF7 (F9G2P) Rabbit mAb recognizes endogenous levels of total SRSF7 protein. Based on the sequence of the peptide antigen, this antibody is expected to detect isoforms 1-4 of SRSF7.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro86 of human SRSF7 protein.

    Background

    The serine/arginine-rich family of splicing factors are crucial regulators in precursor RNA splicing, a process that is associated with maintenance of genome stability, regulation of mRNA export and stability, and translation (1).

    Serine/arginine-rich splicing factor 7 (SRSF7/SFRS7), also known as splicing factor 9G8, is overexpressed in colon and lung cancer, and regulates proliferation and apoptosis through the splicing of Fas mRNA in human colon and lung cancer cells (2). In human colon cancer cells, SRSF7 knockdown increased levels of the CDK inhibitor p21 Waf1/Cip1 in a p53-independent manner (3). SRSF7 also regulates age-dependent splicing in mice (4) and induces expression of cellular senescence markers in response to DNA damage by generating an alternatively spliced form of p53, p53β (5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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