SSEA4 (MC813) Mouse mAb #4755
- IF
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | N/A |
| Source/Isotype | Mouse IgG3 |
Application Key:
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Immunofluorescence (Immunocytochemistry) | 1:100 - 1:400 |
| Flow Cytometry (Fixed/Permeabilized) | 1:200 - 1:800 |
| Flow Cytometry (Live) | 1:200 - 1:800 |
Storage
Protocol
Specificity / Sensitivity
SSEA4 (MC813) Mouse mAb detects endogenous levels of SSEA4 antigen.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by injecting animals with human embryonal carcinoma 2102Ep cl.2A6 cells.
Background
SSEA4 (stage-specific embryonic antigen 4) is a glycolipid carbohydrate epitope expressed on the surface of human teratocarcinoma stem cells, human embryonic germ cells, and human embryonic stem cells (1). Expression of human SSEA4 decreases following differentiation of human embryonal carcinoma cells. Expression of the SSEA4 antigen is absent in murine pluripotent cells, but increases following differentiation (1,2).
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