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Dec 31st Santa Cruz will discontinue a
large number of polyclonal products as
a result of the USDA settlement that was
made public May 19th.

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PhosphoSitePlus® Resource

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Stat1 Antibody 9172 20 µl
Western Blotting Immunoprecipitation Chromatin Immunoprecipitation
H M R Mk 84, 91 Rabbit 
Stat3 (79D7) Rabbit mAb 4904 20 µl
Western Blotting Immunoprecipitation Chromatin Immunoprecipitation
H M R Mk 79, 86 Rabbit IgG
Stat5 Antibody 9363 20 µl
Western Blotting Immunoprecipitation Chromatin Immunoprecipitation
H M R Mk 90 Rabbit 
Stat6 (D3H4) Rabbit mAb 5397 20 µl
Western Blotting Immunoprecipitation Chromatin Immunoprecipitation
H M R 110 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
Goat 

Product Description

The Stat Antibody Sampler Kit provides an economical means to examine multiple Stat proteins: Stat1, Stat3, Stat5 and Stat6. The kit contains enough primary and secondary antibodies to perform two Western blot experiments.


Specificity / Sensitivity

Each Stat antibody in the kit recognizes only its target protein, independent of phosphorylation state.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to the sequence of human Stat1 and the amino-terminal sequence of human Stat5. Antibodies are purified by protein A and peptide affinity chromatography. Stat6 (D3H4) Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly582 of human Stat6 protein. Stat3 (79D7) Rabbit mAb is produced by immunizing animals with GST-Stat3 fusion protein corresponding to the carboxy-terminal sequence of mouse Stat3 protein.

Jaks (Janus Kinases) and Stats (Signal Transducers and Activators of Transcription) are utilized by receptors for a wide variety of ligands including cytokines, hormones, growth factors and neurotransmitters. Jaks, activated via autophosphorylation following ligand-induced receptor aggregation, phosphorylate tyrosine residues on associated receptors, Stat molecules and other downstream signaling proteins (1,2). The phosphorylation of Stat proteins at conserved tyrosine residues activates SH2-mediated dimerization followed rapidly by nuclear translocation. Stat dimers bind to IRE (interferon response element) and GAS (gamma interferon-activated sequence) DNA elements, resulting in the transcriptional regulation of downstream genes (1,2). The remarkable range and specificity of responses regulated by the Stats is determined in part by the tissue-specific expression of different cytokine receptors, Jaks and Stats (2,3), and by the combinatorial coupling of various Stat members to different receptors. Serine phosphorylation in the carboxy-terminal transcriptional activation domain has been shown to regulate the function of Stat1, -2, -3, -4 and -5 (1). Phosphorylation of Stat3 at Ser727 via MAPK or mTOR pathways is required for optimal transcriptional activation in response to growth factors and cytokines including IFN-gamma and CNTF (4,5). Jak/Stat pathways also play important roles in oncogenesis, tumor progression, angiogenesis, cell motility, immune responses and stem cell differentiation (6-11).


1.  Darnell, J.E. et al. (1994) Science 264, 1415-21.

2.  Leonard, W.J. and O'Shea, J.J. (1998) Annu Rev Immunol 16, 293-322.

3.  Caldenhoven, E. et al. (1996) J. Biol. Chem. 271, 13221-13227.

4.  Wen, Z. et al. (1995) Cell 82, 241-50.

5.  Yokogami, K. et al. (2000) Curr Biol 10, 47-50.

6.  Lim, C.P. and Cao, X. (1999) J. Biol. Chem. 274, 31055-31061.

7.  Bromberg, J.F. et al. (1999) Cell 98, 295-303.

8.  Su, L. et al. (1999) J. Biol. Chem. 274, 31770-31774.

9.  Dentelli, P. et al. (1999) J Immunol 163, 2151-9.

10.  Cattaneo, E. et al. (1999) Trends Neurosci. 22, 365-369.

11.  Frank, D.A. (1999) Mol Med 5, 432-56.



For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.

9939
Stat Antibody Sampler Kit