Synaptotagmin-1 (D33B7) Rabbit mAb #14558
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 60 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Synaptotagmin-1 (D33B7) Rabbit mAb recognizes endogenous levels of total synaptotagmin-1 protein. This antibody may also cross-react with an unidentified protein of approximately 45 kDa.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg400 of human synaptotagmin-1 protein.
Background
Synaptotagmin 1 (SYT1) is an integral membrane protein found in synaptic vesicles thought to play a role in vesicle trafficking and exocytosis (1). Individual SYT1 proteins are composed of an amino-terminal transmembrane region, a central linker region and a pair of carboxy-terminal C2 domains responsible for binding Ca2+ (2). The C2 domains appear to be functionally distinct, with the C2A domain responsible for regulating synaptic vesicle fusion in a calcium-dependent manner during exocytosis while the C2B domain allows for interaction between adjacent SYT1 proteins (3). Because synaptotagmin 1 binds calcium and is found in synaptic vesicles, this integral membrane protein is thought to act as a calcium sensor in fast synaptic vesicle exocytosis. Evidence suggests possible roles in vesicle-mediated endocytosis and glucose-induced insulin secretion as well (4,5). SYT1 binds several different SNARE proteins during calcium-mediated vesicle endocytosis and an association between SYT1 and the SNARE protein SNAP-25 is thought to be a key element in vesicle-mediated exocytosis (6).
- Fukuda, M. and Mikoshiba, K. (2001) Biochem Biophys Res Commun 281, 1226-33.
- Südhof, T.C. (2002) J Biol Chem 277, 7629-32.
- Fernández-Chacón, R. et al. (2001) Nature 410, 41-9.
- Lynch, K.L. et al. (2007) Mol Biol Cell 18, 4957-68.
- Gauthier, B.R. and Wollheim, C.B. (2008) Am J Physiol Endocrinol Metab 295, E1279-86.
- Bai, J. et al. (2004) Neuron 41, 929-42.
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