Render Target: STATIC
Render Timestamp: 2024-10-04T09:47:36.557Z
Commit: f04ddd7fea9fb3592f59f61482fcb94610d25cbe
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Syntaxin-4 (E6W7B) Rabbit mAb #67657

Filter:
  • WB
  • IP
  • IF

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 35
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Immunofluorescence (Immunocytochemistry) 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Syntaxin-4 (E6W7B) Rabbit mAb recognizes endogenous levels of total Syntaxin-4 protein. This antibody may also detect a putative SDS-resistant Syntaxin-4 protein-containing (SNARE) complex at approximately 65 kDa.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human Syntaxin-4 protein.

    Background

    Proteins in the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex are integral membrane proteins involved in intracellular vesicle trafficking and exocytosis, and are essential for processes that require rapid, targeted, and regulated membrane fusion such as cell growth, hormone secretion, and neurotransmission (reviewed in 1,2). Functioning as a target or t-SNARE protein, Syntaxin-4 is characterized by a long cytoplasmic N-terminal region encompassing two coiled-coil domains, a single transmembrane domain, and a very short extracellular C-terminus (3). Syntaxin-4 is known to regulate glucose uptake in skeletal muscle and adipose tissue by facilitating the translocation of the glucose transporter GLUT4 from an intracellular compartment to the plasma membrane (4,5). Moreover, human islet β-cells overexpressing Stx4 exhibited enhanced insulin secretory capability, resilience against proinflammatory cytokine-induced apoptosis, and reduced chemokine expression (6). Additionally, research studies have indicated that Syntaxin-4 plays a role in the exocytosis of AMPA receptors in hippocampal neurons (7), lytic granule exocytosis in cytotoxic T-lymphocytes (8), and hepatitis C viral particle release (9).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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