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27112
T-bet/TBX21 (D6N8B) XP® Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

T-bet/TBX21 (D6N8B) XP® Rabbit mAb (BSA and Azide Free) #27112

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  1. WB
  2. IHC
  3. IF
Western Blotting Image 1: T-bet/TBX21 (D6N8B) XP® Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from NK-92 and K-562 cells using T-bet/TBX21 (D6N8B) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: T-bet/TBX21 (D6N8B) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded Non-Hodgkin B-cell lymphoma using T-bet/TBX21 (D6N8B) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: T-bet/TBX21 (D6N8B) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using T-bet/TBX21 (D6N8B) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: T-bet/TBX21 (D6N8B) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human tonsil using T-bet/TBX21 (D6N8B) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: T-bet/TBX21 (D6N8B) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded MJ [G11] cell pellet (left, positive) or K-562 cell pellet (right, negative) using T-bet/TBX21 (D6N8B) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: T-bet/TBX21 (D6N8B) XP® Rabbit mAb (BSA and Azide Free)
Immunofluorescent analysis of NK-92 cells (left, positive) and K-562 cells (right, negative) using T-bet/TBX21 XP® (D6N8B) Rabbit mAb (green) and DRAQ5® #4084 (fluorescent DNA dye). Data were generated using the standard formulation of this product.
To Purchase # 27112
Cat. # Size Qty. Price
27112SF
100 µg $ 746

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 58-68
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #13232. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free. For standard formulation of this product see product #13232.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

T-bet/TBX21 (D6N8B) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total T-bet/TBX21 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly465 of human T-bet/TBX21 protein.

Background

The T-box gene family consists of transcription factors characterized by a related DNA-binding domain (T-box) of approximately 200 amino acids (1,2). The T-box genes exhibit diverse temporal and spatial patterns in the developing embryo. Studies have demonstrated members of this family play crucial roles during embryogenesis in a wide range of organisms by regulating cell fate decisions to establish the early body plan and to regulate later processes underlying organogenesis (3-5). Mutations in T-box genes are associated with many developmental defects (6). Recent studies also indicate potential roles in cancer by members of the T-box family (7-9).
  1. Wilkinson, D.G. et al. (1990) Nature 343, 657-9.
  2. Papaioannou, V.E. and Silver, L.M. (1998) Bioessays 20, 9-19.
  3. Showell, C. et al. (2004) Dev Dyn 229, 201-18.
  4. Papaioannou, V.E. (2001) Int Rev Cytol 207, 1-70.
  5. Hoogaars, W.M. et al. (2007) Cell Mol Life Sci 64, 646-60.
  6. Baldini, A. (2004) Curr Opin Cardiol 19, 201-4.
  7. Abrahams, A. et al. (2010) IUBMB Life 62, 92-102.
  8. Rowley, M. et al. (2004) J Mammary Gland Biol Neoplasia 9, 109-18.
  9. Yang, X.R. et al. (2009) Nat Genet 41, 1176-8.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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