T Cell Signaling Antibody Sampler Kit #9382
Inquiry Info. # 9382
Please see our recommended alternatives.
Product Information
Kit Usage Information
Protocols
- 2717: Western Blotting, Immunofluorescence, Flow
- 2751: Western Blotting, Immunoprecipitation (Agarose)
- 2787: Western Blotting
- 3165: Western Blotting, Immunoprecipitation (Agarose), Immunofluorescence, Flow
- 3584: Western Blotting, Immunoprecipitation (Magnetic)
- 4958: Western Blotting, Immunoprecipitation (Magnetic)
- 7074: Western Blotting
- 9166: Western Blotting, Immunohistochemistry (Paraffin), Flow
Product Description
Background
LAT, a transmembrane adaptor protein expressed in T, NK and mast cells, is an important mediator for T cell receptor (TCR) signaling (9). Upon TCR engagement, activated Zap-70 phosphorylates LAT at multiple conserved tyrosine residues within SH2 binding motifs, exposing these motifs as the docking sites for downstream signaling targets (10,11). The phosphorylation of LAT at Tyr171 and 191 enables the binding of Grb2, Gads/SLP-76, PLCgamma1 and PI3 kinase through their SH2 domain and translocates them to the membrane. This process eventually leads to activation of the corresponding signaling pathways (11-12).
- Chu, D.H. et al. (1998) Immunol Rev 165, 167-80.
- Iwashima, M. et al. (1994) Science 263, 1136-9.
- Neumeister, E.N. et al. (1995) Mol Cell Biol 15, 3171-8.
- Chan, A.C. et al. (1995) EMBO J 14, 2499-508.
- Williams, B.L. et al. (1999) EMBO J 18, 1832-44.
- Di Bartolo, V. et al. (1999) J Biol Chem 274, 6285-94.
- Wiestner, A. et al. (2003) Blood 101, 4944-51.
- Crespo, M. et al. (2003) N Engl J Med 348, 1764-75.
- Wonerow, P. and Watson, S.P. (2001) Oncogene 20, 6273-83.
- Zhang, W. et al. (1998) Cell 92, 83-92.
- Paz, P.E. et al. (2001) Biochem J 356, 461-71.
- Zhang, W. et al. (2000) J Biol Chem 275, 23355-61.
Alternate Names
antigen receptor; LAT; Lck; lymphocyte; SLP76; syk; T cell; TCR; Zap-70; Zap70
Limited Uses
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