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TAF15 Antibody #13150

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  • WB

Inquiry Info. # 13150

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    Product Specifications

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 77
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    TAF15 Antibody recognizes endogenous levels of total TAF15 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Hamster, Bovine, Dog, Pig, Horse

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human TAF15 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    TATA-binding protein (TBP)-associated factor 15 (TAF15) is a member of the multifunctional FUS, EWS, and TAF15 (FET) family of proteins that bind both DNA and RNA and may be important for transcriptional regulation and RNA processing (1,2). Research studies have shown that FET family proteins, including TAF15, can be found as oncogenic fusion proteins with transcription factors in various cancers. The fusion protein consists of the FET protein amino-terminal transcription activation domain and the transcription factor DNA binding domain, creating an aberrant transcription factor with potent transactivation potential (1-3). TAF15 can be part of a subset of the transcription factor IID (TFIID) complex, indicating that it may form a transcription pre-initiation complex at active genes, even if it is not considered a canonical TAF (4,5). Additional studies suggest roles for TAF15 in RNA splicing through its association with U1 small nuclear RNA and in regulation of cell cycle regulatory gene expression through a mechanism involving miRNA biosynthesis (6,7). TAF15 aggregation and amyloid filament formation have been reported within the brain of individuals with frontotemporal lobar degeneration-FUS (FTLD-FUS) (8).

    Alternate Names

    68 kDa TATA-binding protein-associated factor; hTAFII68; Npl3; RBP56; RBP56/CSMF fusion; RNA-binding protein 56; TAF(II)68; TAF15; TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa; TAF2N; TAFII68; TATA box binding protein (TBP)-associated factor, RNA polymerase II, N, 68kD (RNA-binding protein 56); TATA box-binding protein-associated factor 2N (RNA-binding protein 56); TATA-binding protein-associated factor 2N; TATA-box binding protein associated factor 15; TBP-associated factor 15

    For Research Use Only. Not for Use in Diagnostic Procedures.
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