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TAP2 (E8G5I) Rabbit Monoclonal Antibody #25657

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  • WB
  • IP

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 72
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    TAP2 (E8G5I) Rabbit Monoclonal Antibody recognizes endogenous levels of total TAP2 protein. This antibody does not cross-react with TAP1 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val485 of human TAP2 protein.

    Background

    CD8+ cytotoxic T cells recognize peptides presented by MHC class I molecules on the surface of infected cells and tumor cells. The transporters associated with antigen processing 1 and 2 (TAP1 and TAP2) form the TAP complex, which resides on the ER membrane and transports peptides from the cytoplasm into the ER for loading onto MHC class I molecules (1-8). In addition, TAP localized to endosomal membranes is important for cross-presentation by dendritic cells (9,10). IFN-γ produced by T cells and NK cells in response to infection causes upregulation of TAP1 and TAP2, resulting in increased antigen presentation to T cells (11). Some viral proteins inhibit TAP function or downregulate TAP expression, resulting in viral immune evasion (12,13). In addition, investigators have observed reduced TAP expression in a variety of tumor types, and it is thought to be one mechanism for tumor immune evasion (14).

    Alternate Names

    ABC transporter, MHC 2; ABC-transporter; ABC18; ABCB3; Antigen peptide transporter 2; APT2; ATP-binding cassette sub-family B member 3; ATP-binding cassette, sub-family B (MDR/TAP), member 3; D6S217E; peptide supply factor 2; peptide transporter involved in antigen processing 2; peptide transporter PSF2; peptide transporter TAP2; PSF-2; PSF2; really interesting new gene 11 protein; RING11; TAP2; transporter 2, ABC (ATP binding cassette); transporter 2, ATP binding cassette subfamily B member; transporter 2, ATP-binding cassette, sub-family B; transporter 2, ATP-binding cassette, sub-family B (MDR/TAP); Y1

    For Research Use Only. Not for Use in Diagnostic Procedures.
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