Render Target: STATIC
Render Timestamp: 2024-12-02T11:18:46.223Z
Commit: cd2fae6ca3f811b1ddb1df24ac291ed56d5d501b
XML generation date: 2024-09-30 01:56:06.113
Product last modified at: 2024-09-30T08:00:47.524Z
1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

TDRD3 (D3O2G) Rabbit mAb #5942

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 83
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    TDRD3 (D3O2G) Rabbit mAb recognizes endogenous levels of total TDRD3 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human TDRD3 protein.

    Background

    Tudor domain-containing protein 3 (TDRD3) contains a tudor domain through which it binds to asymmetric di-methyl histone H3 (Arg17) and asymmetric di-methyl histone H4 (Arg3). Both of these histone marks are associated with transcription activation (1,2). TDRD3 is targeted to estrogen responsive genes where it performs as a coactivator (1). TDRD3 acts as a scaffolding protein to recruit Topoisomerase III B (TOP3B) to target genes to reduce transcription-generated R loops by relaxing negatively supercoiled DNA thereby reducing genomic instability (3). In addition to the nucleus, TDRD3 also resides in the cytoplasm where it associates with actively translating polyribosomes and accumulates into stress granules upon exposure to stress, implicating its role in post-transcriptional regulation of RNA (2,4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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