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TEX264 (E2J3J) Rabbit Monoclonal Antibody #44830

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  • WB
  • IP

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 45
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    TEX264 (E2J3J) Rabbit Monoclonal Antibody recognizes endogenous levels of total TEX264 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro193 of human TEX264 protein.

    Background

    TEX264 (testis expressed gene 264) is a single-pass transmembrane protein localized to the endoplasmic reticulum (ER) that plays a key role in the clearance of damaged ER via the selective autophagic process of ER-phagy (1,2). It contains an LC3 interacting region (LIR) acting as a bridge from the ER to Atg8 family members, LC3 and GABARAP, on the autophagosome. Phosphorylation of TEX264 by casein kinase 2 can enhance its interaction with Atg8 family members (3). TEX264-mediated ER-phagy has been observed in pathological conditions, including acetaminophen-induced hepatotoxicity and in neuroinflammation associated with Alzheimer’s Disease (4,5). TEX264 has also been found to contribute to genomic stability through association with the VCP ATPase promoting TOP1-mediated DNA repair (6).

    Alternate Names

    DKFZp451H0417; FLJ13935; Putative secreted protein Zsig11; SIG11; testis expressed 264; testis expressed 264, ER-phagy receptor; testis expressed gene 264; testis expressed sequence 264; Testis-expressed protein 264; Testis-expressed sequence 264 protein; TEX264; TX264; ZSIG11

    For Research Use Only. Not for Use in Diagnostic Procedures.
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