Render Target: STATIC
Render Timestamp: 2025-03-20T10:32:20.020Z
Commit: 779953b12a5930618aae6aca7c87fb286faeb1d7
XML generation date: 2025-03-07 13:16:47.334
Product last modified at: 2025-01-01T09:04:48.320Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

TFB2M (E5P6W) Rabbit mAb #50837

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 38
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    TFB2M (E5P6W) Rabbit mAb recognizes endogenous levels of total TFB2M protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu388 of human TFB2M protein.

    Background

    Transcription factor A of the mitochondria (TFAM) and transcription factor B2 of the mitochondria (TFB2M) are critical components of the mitochondrial RNA polymerase machinery (1,2). TFB2M binds to POLRMT and induces structural changes, enabling promoter opening (3,4). The ability of TFB2M to bind at mtDNA promoters is regulated by phosphorylation (5). TFB2M has also been shown to have methyltransferase activity towards rRNA (6). TFB2M can be overexpressed in hepatocellular carcinoma, where it promotes growth and metastasis through ROS-Akt-NF-κB signaling (7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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