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Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

TFCP2 Antibody recognizes endogenous levels of total TFCP2 protein.

Species predicted to react based on 100% sequence homology: Bovine, Dog, Horse

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu61 of human TFCP2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

The transcription factor CP2 (TFCP2, LSF) is a ubiquitous nuclear protein that was initially shown to bind and activate the alpha-globin promoter in erythroid cells (1). Research studies show that TFCP2 functions as an oncogene in hepatocellular carcinoma (HCC) cells. Overexpression of TFCP2 is seen in HCC patient samples and cell lines; TFCP2 expression correlates with high tumor grade and poor prognosis (2). Forced expression of TFCP2 in less aggressive HCC cells results in highly aggressive, angiogenic and metastatic tumors, while inhibition of TFCP2 abrogates growth and metastasis of highly aggressive HCC cells (2). Additional studies show that TFCP2 acts downstream of Notch1 in HCC cells, where it mediates Notch pathway signaling during proliferation and invasion of hepatocellular carcinoma (3). TFCP2 functions as an oncogene as it upregulates multiple genes involved in angiogenesis, cell invasion, and chemoresistance, including osteopontin, metalloproteinase-9, fibronectin 1, tight junction protein 1, and thymidylate synthase (2-5). Factor quinolinone inhibitor 1 (FQI1) is a small molecule inhibitor of TFCP2 that inhibits TFCP2 DNA-binding activity, reduces expression of TFCP2 target genes, and rapidly induces cell death in TFCP2-overexpressing HCC cell lines (6).

1.  Lim, L.C. et al. (1993) J Biol Chem 268, 18008-17.

2.  Yoo, B.K. et al. (2010) Proc Natl Acad Sci U S A 107, 8357-62.

3.  Fan, R.H. et al. (2011) World J Gastroenterol 17, 3420-30.

4.  Santhekadur, P.K. et al. (2012) J Biol Chem 287, 3425-32.

5.  Xu, X. et al. (2015) J Exp Clin Cancer Res 34, 6.

6.  Grant, T.J. et al. (2012) Proc Natl Acad Sci U S A 109, 4503-8.

Entrez-Gene Id 7024
Swiss-Prot Acc. Q12800

For Research Use Only. Not For Use In Diagnostic Procedures.
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TFCP2 Antibody