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PhosphoPlus® TFEB (Ser211) Antibody Duet
Primary Antibodies
Antibody Duet

PhosphoPlus® TFEB (Ser211) Antibody Duet #48590

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Western blot analysis of extracts from Raji, Daudi, and HDLM-2 cell lines, untreated (-) or treated with Torin 1 #14379 (250 nM, 5 hr), using Phospho-TFEB (Ser211) (E9S8N) Rabbit mAb (upper) or TFEB Antibody #4240 (lower).
Western blot analysis of extracts from various cell lines using TFEB (D2O7D) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from 293T cells, untransfected (-) or transfected with a construct expresssing full-length human TFEB (hTFEB; +) using TFEB (D2O7D) Rabbit mAb.
Immunoprecipitation of TFEB from Raji cell extracts. Lane 1 represents 10% input, lane 2 is immunoprecipitated with Rabbit (DA1E) mAb IgG XP® Isotype control #3900, and lane 3 is TFEB (D2O7D) Rabbit mAb. Western blot was performed using TFEB (D2O7D) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using TFEB (D2O7D) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lymphoma using TFEB (D2O7D) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using TFEB (D2O7D) Rabbit mAb.
Chromatin immunoprecipitations were performed with cross-linked chromatin from Raji cells treated with Torin-1 (250 nM, 5 hr) and either TFEB (D2O7D) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human GLA Promoter Primers #27131, human ATP6V1H promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
To Purchase # 48590
Cat. # Size Qty. Price
1 Kit

Product Includes Quantity Reactivity MW(kDa) Isotype
Phospho-TFEB (Ser211) (E9S8N) Rabbit mAb 37681 100 µl H 70 Rabbit IgG
TFEB (D2O7D) Rabbit mAb 37785 100 µl H 65-70 Rabbit IgG

Product Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.


Transcription factor EB (TFEB) is a member of the Myc-related, bHLH leucine-zipper family of transcription factors that drives the expression of a network of genes known as the Coordinated Lysosomal Expression and Regulation (CLEAR) network (1,2). TFEB specifically recognizes and binds regulatory sequences within the CLEAR box (GTCACGTGAC) of lysosomal and autophagy genes, resulting in the upregulated expression of genes involved in lysosome biogenesis and function, and regulation of autophagy (1,2). TFEB is activated in response to nutrient deprivation, stimulating translocation to the nucleus where it forms homo- or heterooligomers with other members of the microphthalmia transcription factor (MiTF) subfamily and resulting in upregulation of autophagosomes and lysosomes (3-5). Recently, it has been shown that TFEB is a component of mammalian target of rapamycin (mTOR) complex 1 (mTORC1), which regulates the phosphorylation and nuclear translocation of TFEB in response to cellular starvation and stress (6-9). During normal growth conditions, TFEB is phosphorylated at Ser211 in an mTORC1-dependent manner. Phosphorylation promotes association of TFEB with 14-3-3 family proteins and retention in the cytosol. Inhibition of mTORC1 results in a loss of TFEB phosphorylation, dissociation of the TFEB/14-3-3 complex, and rapid transport of TFEB to the nucleus where it increases transcription of CLEAR and autophagy genes (10). TFEB has also been shown to be activated in a nutrient-dependent manner by p42 MAP kinase (Erk2). TFEB is phosphorylated at Ser142 by Erk2 in response to nutrient deprivation, resulting in nuclear localization and activation, and indicating that pathways other than mTOR contribute to nutrient sensing via TFEB (3).

Limited Uses

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