Render Target: STATIC
Render Timestamp: 2024-10-15T09:57:24.570Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-10-15 04:01:06.794
Product last modified at: 2024-10-15T07:00:55.325Z
1% for the planet logo
PDP - Template Name: Antibody Sampler Kit
PDP - Template ID: *******4a3ef3a

TFEB Signaling Antibody Sampler Kit #63734

    Product Information

    Product Description

    The TFEB Signaling Antibody Sampler Kit provides an economical means of analyzing the regulation of TFEB. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

    Specificity / Sensitivity

    Each antibody in the TFEB Signaling Antibody Sampler Kit detects endogenous levels of its target protein. Pan-Calcineurin A Antibody detects endogenous levels of the alpha isoform of Calcineurin A. It may also recognize the beta and gamma isoforms of Calcineurin A. The antibody does not cross-react with protein phosphatase 1 or 2A.

    Source / Purification

    Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Gly412 of human TFEB and Ser2481 of human mTOR, and synthetic phosphopeptides corresponding to residues surrounding Ser122 and Ser211 of human TFEB and Ser2448 of human mTOR. Polyclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human Calcineurin A (alpha isoform). Polyclonal antibodies are purified by Protein A and peptide affinity chromatography.

    Background

    Transcription factor EB (TFEB) is a member of the Myc-related, bHLH leucine-zipper family of transcription factors that drives the expression of a network of genes known as the Coordinated Lysosomal Expression and Regulation (CLEAR) network (1,2). TFEB specifically recognizes and binds regulatory sequences within the CLEAR box (GTCACGTGAC) of lysosomal and autophagy genes, resulting in the upregulated expression of genes involved in lysosome biogenesis and function, and regulation of autophagy (1,2). TFEB is activated in response to nutrient deprivation, stimulating translocation to the nucleus where it forms homo- or heterooligomers with other members of the microphthalmia transcription factor (MiTF) subfamily and resulting in upregulation of autophagosomes and lysosomes (3-5). Recently, it has been shown that TFEB is a component of mammalian target of rapamycin (mTOR) complex 1 (mTORC1), which regulates the phosphorylation and nuclear translocation of TFEB in response to cellular starvation and stress (6-9). During normal growth conditions, TFEB is phosphorylated at Ser211 in an mTORC1-dependent manner. Phosphorylation promotes association of TFEB with 14-3-3 family proteins and retention in the cytosol. Inhibition of mTORC1 results in a loss of TFEB phosphorylation, dissociation of the TFEB/14-3-3 complex, and rapid transport of TFEB to the nucleus where it increases transcription of CLEAR and autophagy genes (10). TFEB has also been shown to be activated in a nutrient-dependent manner by p42 MAP kinase (Erk2). TFEB is phosphorylated at Ser142 by Erk2 in response to nutrient deprivation, resulting in nuclear localization and activation, and indicating that pathways other than mTOR contribute to nutrient sensing via TFEB (3).
    Additional studies have also identified phosphorylation of TFEB at Ser122 that is dependent on mTORC1 (11). mTOR activity is associated with phosphorylation at Ser2448 via the PI3 kinase/Akt signaling pathway (12). Lysosomal calcium release activates the phosphatase calcineurin that dephosphorylates TFEB and promotes nuclear translocation and autophagy (13).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    XP is a registered trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.