Thyroid Transcription Factor 1 (TTF-1) (D2E8) Rabbit mAb #12373
- WB
- IF
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 39, 42 |
| Source/Isotype | Rabbit |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Frozen) | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:50 |
Storage
For a carrier free (BSA and azide free) version of this product see product #19428.
Protocol
Specificity / Sensitivity
Thyroid Transcription Factor 1 (TTF-1) (D2E8) Rabbit mAb recognizes endogenous levels of total thyroid transcription factor 1 protein.
Species Reactivity:
Human, Mouse, Rat
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human thyroid transcription factor 1 protein.
Background
Thyroid Transcription Factor 1 (TTF-1, also known as NKX2-1), a member of the NKX homeobox transcription factor family, was initially discovered in the FRTL-5 rat thyroid cell line (1). Subsequent studies have shown that TTF-1 plays an important role in differentiation and morphogenesis of the developing thyroid, lung, and ventral forebrain (2). TTF-1 controls the expression of several genes, some of which are tissue specific, such as: thyroglobulin, thyroperoxidase, and the thyrotropin receptor in the thyroid; and surfactant proteins and clara cell secretory protein in the lung (2,3). Investigators have found that TTF-1 is expressed in malignant tumors of the thyroid and lung, and it is commonly used as a marker for both primary and malignant lung cancers (4-6).
- Civitareale, D. et al. (1989) EMBO J 8, 2537-42.
- Boggaram, V. (2009) Clin Sci (Lond) 116, 27-35.
- Yamaguchi, T. et al. (2012) Cancer Cell 21, 348-61.
- Whithaus, K. et al. (2012) Arch Pathol Lab Med 136, 155-62.
- Yoshida, A. et al. (2011) Lung Cancer 72, 309-15.
- Moldvay, J. et al. (2004) Pathol Oncol Res 10, 85-8.
Limited Uses
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