Render Target: STATIC
Render Timestamp: 2024-11-01T09:49:40.826Z
Commit: 23cb9f61fe67e1e9093fd644a533c4ff516a6463
XML generation date: 2024-09-30 01:55:22.768
Product last modified at: 2024-10-02T17:00:09.049Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Tie2 (AB33) Mouse mAb #4224

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H B
    SENSITIVITY Endogenous
    MW (kDa) 160
    Source/Isotype Mouse IgG1
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • B-Bovine 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Tie2 (AB33) Mouse mAb detects endogenous levels of Tie2 in various endothelial cell lines. It does not cross-react with related proteins.

    Species Reactivity:

    Human, Bovine

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant Tie2 protein fragments corresponding to the extracellular domain of human Tie2.

    Background

    Tie2/Tek is a receptor tyrosine kinase (RTK) expressed almost exclusively on endothelial cells. It is critical for vasculogenesis and could be important for maintaining endothelial cell survival and integrity in adult blood vessels as well as tumor angiogenesis (1-3). A family of ligands known as the angiopoietins binds to Tie2. Interestingly, these ligands appear to have opposing actions; Angiopoietin-1 (Ang1) and Angiopoietin-4 (Ang4) stimulate tyrosine phosphorylation of Tie2; Angiopoietin-2 (Ang2) and Angiopoietin-3 (Ang3) can inhibit this phosphorylation (4,5). Downstream signaling components, including Grb2, Grb7, Grb14, SHP-2, the p85 subunit of phosphatidylinositol 3-kinase, and p56/Dok-2 interact with Tie2 in a phosphotyrosine-dependent manner through their SH2 or PTB domains (6,7). Tyr992 is located on the putative activation loop of Tie2 and is a major autophosphorylation site (8).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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