TMOD3 (F9M4D) Rabbit mAb #52405
- WB
- IP
- IF
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 40 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:1600 - 1:6400 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
TMOD3 is widely expressed and, like other family members, binds tropomyosin and actin filaments (F-actin). This functions in the capping process (5). However, unlike other family members, TMOD3 is also able to bind to actin monomer (6). TMOD3 localizes to the lateral cell membrane in polarized epithelial cells and regulates cell shape (7). Loss of TMOD3 in mice leads to embryonic lethality with an impaired erythroid differential in the fetal liver (8). TMOD3 is uniquely phosphorylated by Akt2 upon insulin stimulation and regulates Glut4 trafficking through actin remodeling (9). Expression of TMOD3 may promote tumor progression, including liver cancer (10). Studies have also found a ferroptosis-related gene signature in lung adenocarcinoma that includes TMOD3 (11).
- Fischer, R.S. and Fowler, V.M. (2003) Trends Cell Biol 13, 593-601.
- Rao, J.N. et al. (2014) Science 345, 463-7.
- Ito-Kureha, T. et al. (2015) Cancer Res 75, 62-72.
- Agnelli, L. et al. (2012) Blood 120, 1274-81.
- Parreno, J. and Fowler, V.M. (2018) Biophys Rev 10, 1605-1615.
- Fischer, R.S. et al. (2006) J Biol Chem 281, 36454-65.
- Weber, K.L. et al. (2007) J Cell Sci 120, 3625-32.
- Sui, Z. et al. (2014) Blood 123, 758-67.
- Lim, C.Y. et al. (2015) Nat Commun 6, 5951.
- Jin, C. et al. (2019) Oncol Rep 41, 3060-3068.
- Tian, W. et al. (2024) J Gene Med 26, e3579.
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