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  3. Toll-like Receptor 4 Antibody

Toll-like Receptor 4 Antibody #2246

Inquiry Info. # 2246

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    Product Specifications

    REACTIVITY
    SENSITIVITY
    MW (kDa) 90
    SOURCE Rabbit

    Product Information

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Specificity / Sensitivity

    Toll-like Receptor 4 Antibody detects endogenous levels of total TLR4 protein. Cross reactivity was not detected with other family members at physiological conditions.

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding serine 681 of human TLR4. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses (1-4). TLRs recognize conserved motifs found in various pathogens and mediate defense responses (5-7). Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes (4). The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the Toll/Interleukin-1 receptor (TIR) domain (1). Upon activation, TLRs associate with a number of cytoplasmic adapter proteins containing TIR domains, including myeloid differentiation factor 88 (MyD88), MyD88-adapter-like/TIR-associated protein (MAL/TIRAP), TIR domain-containing adapter-inducing IFN-β (TRIF), and Toll-receptor-associated molecule (TRAM) (8-10). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK (8,11-14). Activation of IKK leads to the degradation of IκB, which normally maintains NF-κB in an inactive state by sequestering it in the cytoplasm.

    Alternate Names

    ARMD10; CD284; homolog of Drosophila toll; hToll; TLR-4; TLR4; TOLL; toll like receptor 4; toll like receptor 4 protein; Toll-like receptor 4

    Database Links

    UniProt ID: O00206


    Entrez-Gene Id: 7099


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