Toll-like Receptor 7 (E4J3Z) Rabbit mAb #82658
- WB
- IP
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 74, 120, 140 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Protocol
Specificity / Sensitivity
Toll-like Receptor 7 (E4J3Z) Rabbit mAb recognizes endogenous levels of total TLR7 protein, including the full-length protein that migrates at 140 kDa, the full-length deglycosylated protein that migrates at 120 kDa, and the C-terminal cleaved protein that migrates at 74 kDa.
Species Reactivity:
Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment specific to mouse TLR7 protein.
Background
Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses (1-4). TLRs recognize conserved motifs found in various pathogens and mediate defense responses (5-7). Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes (4). The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the Toll/Interleukin-1 receptor (TIR) domain (1). Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains, including myeloid differentiation factor 88 (MyD88), MyD88-adaptor-like/TIR-associated protein (MAL/TIRAP), Toll-receptor-associated activator of interferon (TRIF), and Toll-receptor-associated molecule (TRAM) (8-10). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK (8,11-14). Activation of IKK leads to the degradation of IκB, which normally maintains NF-κB in an inactive state by sequestering it in the cytoplasm.
TLR7, 8 and 9 form a group of structurally related TLR family members that are localized to intracellular endosomes (15-17). TLR7 shows highest expression in lung, placenta, and spleen (15). TLR7 mediates responses to a class of synthetic compounds, including imidazoquinolines, guanosine-based drugs that induce anti-viral responses (18). TLR7 responds to ssRNA viruses to activate NF-κB and trigger IFN production (19-21).
- Akira, S. (2003) J Biol Chem 278, 38105-8.
- Beutler, B. (2004) Nature 430, 257-63.
- Dunne, A. and O'Neill, L.A. (2003) Sci STKE 2003, re3.
- Medzhitov, R. et al. (1997) Nature 388, 394-7.
- Schwandner, R. et al. (1999) J Biol Chem 274, 17406-9.
- Takeuchi, O. et al. (1999) Immunity 11, 443-51.
- Alexopoulou, L. et al. (2001) Nature 413, 732-8.
- Zhang, F.X. et al. (1999) J Biol Chem 274, 7611-4.
- Horng, T. et al. (2001) Nat Immunol 2, 835-41.
- Oshiumi, H. et al. (2003) Nat Immunol 4, 161-7.
- Muzio, M. et al. (1997) Science 278, 1612-5.
- Wesche, H. et al. (1997) Immunity 7, 837-47.
- Suzuki, N. et al. (2002) Nature 416, 750-6.
- Irie, T. et al. (2000) FEBS Lett 467, 160-4.
- Chuang, T.H. and Ulevitch, R.J. (2000) Eur Cytokine Netw 11, 372-8.
- Du, X. et al. (2000) Eur Cytokine Netw 11, 362-71.
- Heil, F. et al. (2003) Eur J Immunol 33, 2987-97.
- Hemmi, H. et al. (2002) Nat Immunol 3, 196-200.
- Heil, F. et al. (2004) Science 303, 1526-9.
- Diebold, S.S. et al. (2004) Science 303, 1529-31.
- Lund, J.M. et al. (2004) Proc Natl Acad Sci U S A 101, 5598-603.
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