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9971
Toll-like Receptor Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Toll-like Receptor Antibody Sampler Kit #9971

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Western blot analysis of extracts from HeLa cells, mock transfected or transfected with human TLR1, using Toll-like Receptor 1 Antibody.

Immunoprecipitation of TLR2 from MUTZ-3 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Toll-like Receptor 2 (D7G9Z) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Toll-like Receptor 2 (D7G9Z) Rabbit mAb.

Western blot analysis of extracts from various cell lines using Toll-like Receptor 7 (D7) Rabbit mAb.

Western blot analysis of extracts from various cell lines using Toll-like Receptor 9 (D2C9) Rabbit mAb.

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western blot analysis of extracts from SR cells using Toll-like Receptor 1 Antibody.

Western blot analysis of THP-1 and MUTZ-3 cells using Toll-like Receptor 2 (D7G9Z) Rabbit mAb.

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with human TLR7 (+), using Toll-like Receptor 7 (D7) Rabbit mAb.

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing HA-tagged full-length human TLR2 (hTLR2-HA; +), using Toll-like Receptor 2 (D7G9Z) Rabbit mAb.

To Purchase # 9971T
Product # Size Price
9971T
1 Kit  (4 x 20 µl) $ 327

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Toll-like Receptor 1 Antibody 2209 20 µl
  • WB
H 86 Rabbit 
Toll-like Receptor 2 (D7G9Z) Rabbit mAb 12276 20 µl
  • WB
  • IP
H 90-105 Rabbit IgG
Toll-like Receptor 7 (D7) Rabbit mAb 5632 20 µl
  • WB
  • IP
H 140 Rabbit IgG
Toll-like Receptor 9 (D2C9) Rabbit mAb 5845 20 µl
  • WB
  • IP
H 130 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Product Description

The Toll-Like Receptor Antibody Sampler Kit is an economical way to examine the total protein levels of a number of toll-like receptors. This kit includes enough primary and secondary antibodies to perform two Western blot experiments with each antibody.

Specificity / Sensitivity

All antibodies contained in this kit detect endogenous levels of their respective total TLR protein. Cross reactivity was not detected with other family members at physiological conditions.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide and are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with recombinant human proteins or synthetic peptides.

Background

Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses (1-4). TLRs recognize conserved motifs found in various pathogens and mediate defense responses (5-7). Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes (4). The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the Toll/Interleukin-1 receptor (TIR) domain (1). Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains, including myeloid differentiation factor 88 (MyD88), MyD88-adaptor-like/TIR-associated protein (MAL/TIRAP), Toll-receptor-associated activator of interferon (TRIF), and Toll-receptor-associated molecule (TRAM) (8-10). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK (8,11-14). Activation of IKK leads to the degradation of IκB, which normally maintains NF-κB in an inactive state by sequestering it in the cytoplasm.

  1. Akira, S. (2003) J Biol Chem 278, 38105-8.
  2. Beutler, B. (2004) Nature 430, 257-63.
  3. Dunne, A. and O'Neill, L.A. (2003) Sci STKE 2003, re3.
  4. Medzhitov, R. et al. (1997) Nature 388, 394-7.
  5. Schwandner, R. et al. (1999) J Biol Chem 274, 17406-9.
  6. Takeuchi, O. et al. (1999) Immunity 11, 443-51.
  7. Alexopoulou, L. et al. (2001) Nature 413, 732-8.
  8. Zhang, F.X. et al. (1999) J Biol Chem 274, 7611-4.
  9. Horng, T. et al. (2001) Nat Immunol 2, 835-41.
  10. Oshiumi, H. et al. (2003) Nat Immunol 4, 161-7.
  11. Muzio, M. et al. (1997) Science 278, 1612-5.
  12. Wesche, H. et al. (1997) Immunity 7, 837-47.
  13. Suzuki, N. et al. (2002) Nature 416, 750-6.
  14. Irie, T. et al. (2000) FEBS Lett 467, 160-4.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.

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