Tox4 (E9U3L) Rabbit mAb (ChIP Formulated) #46419
- ChIP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Chromatin IP | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Tox4 (E9U3L) Rabbit mAb (ChIP Formulated) recognizes endogenous levels of total Tox4 protein. This antibody does not cross-react with human Tox, Tox2, or Tox3 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val583 of human Tox4 protein.
Background
Thymocyte selection-associated high mobility group box protein (Tox) is a DNA-binding nuclear factor and member of the evolutionarily conserved high-motility group (HMG)-box superfamily. Tox also defines a small subfamily of proteins that include Tox2, Tox3, and Tox4, all of which are highly conserved in vertebrate species but have unique tissue expression patterns and functions (1,2).
Tox4 is a regulatory factor of PP1 phosphatases and plays important roles in transcription, which include restricting pause release and early productive elongation, facilitating transcriptional reinitiation, and promoting late productive elongation (3). Tox4 has been shown to modulate cell fate by reprogramming from a somatic state into a pluripotent and neuronal fate (4). Tox4 is a regulator of hepatic glucose production in an insulin receptor and FoxO1-independent manner, and its expression is increased in the liver in diabetic patients with nonalcoholic fatty liver disease (NAFLD) (5).
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