Render Target: STATIC
Render Timestamp: 2024-12-06T11:46:47.612Z
Commit: 224419269841c11382c4555dbee545259bf6c379
XML generation date: 2024-09-30 01:59:07.109
Product last modified at: 2024-09-30T08:02:14.011Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Tox4 (E9U3L) Rabbit mAb (ChIP Formulated) #46419

Filter:
  • ChIP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • ChIP-Chromatin Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
    Application Dilution
    Chromatin IP 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Tox4 (E9U3L) Rabbit mAb (ChIP Formulated) recognizes endogenous levels of total Tox4 protein. This antibody does not cross-react with human Tox, Tox2, or Tox3 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val583 of human Tox4 protein.

    Background

    Thymocyte selection-associated high mobility group box protein (Tox) is a DNA-binding nuclear factor and member of the evolutionarily conserved high-motility group (HMG)-box superfamily. Tox also defines a small subfamily of proteins that include Tox2, Tox3, and Tox4, all of which are highly conserved in vertebrate species but have unique tissue expression patterns and functions (1,2).

    Tox4 is a regulatory factor of PP1 phosphatases and plays important roles in transcription, which include restricting pause release and early productive elongation, facilitating transcriptional reinitiation, and promoting late productive elongation (3). Tox4 has been shown to modulate cell fate by reprogramming from a somatic state into a pluripotent and neuronal fate (4). Tox4 is a regulator of hepatic glucose production in an insulin receptor and FoxO1-independent manner, and its expression is increased in the liver in diabetic patients with nonalcoholic fatty liver disease (NAFLD) (5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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