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TRPC6 (D3G1Q) Rabbit mAb #16716

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Inquiry Info. # 16716

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    Product Specifications

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 110
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    TRPC6 (D3G1Q) Rabbit mAb recognizes endogenous levels of total TRPC6 protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human TRPC6 protein.

    Background

    Transient receptor potential (TRP) canonical 6 (TRPC6) belongs to the superfamily of TRP cation channels. The TRPC subfamily (TRPC1-7) is a group of calcium-permeable cation channels that mediates the increase in intracellular [Ca2+] following activation by G-protein-coupled receptors or receptor tyrosine kinases (1). TRPC6 is directly activated by diacylglycerol (DAG) (2). Various tissues express TRPC6, including brain, lung, kidney, ovary and small intestine. TRPC6 exerts a variety of biological functions in various tissues. In brain, TRPC6 plays important roles in synaptic plasticity, spatial cognition (3) and protects neurons from ischemic excitotoxicity (4). In kidney, TRPC6 is expressed in mesangial cells and podocytes of renal glomeruli (5) and regulates glomerular filtration (6). Mutations in TRPC6 gene cause autosomal dominant focal segmental glomerulosclerosis (7). In smooth muscle cells, TRPC6 mediates Na+ influx followed by Ca2+ entry via Na+/Ca2+ -exchanger (NCX) reversal which leads to contraction (8, 9).

    Alternate Names

    FLJ11098; FLJ14863; focal segmental glomerulosclerosis 2; FSGS2; Short transient receptor potential channel 6; transient receptor potential cation channel subfamily C member 6; transient receptor potential cation channel, subfamily C, member 6; Transient receptor protein 6; TRP-6; TRP6; TRPC6

    For Research Use Only. Not for Use in Diagnostic Procedures.
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