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TRPV4 (E6W1P) Rabbit Monoclonal Antibody

TRPV4 (E6W1P) Rabbit Monoclonal Antibody #91660

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Product Specifications

REACTIVITY	H Mk
SENSITIVITY	Endogenous
MW (kDa)	95-102
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IP-Immunoprecipitation

Species Cross-Reactivity Key:

H-Human
Mk-Monkey

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

TRPV4 (E6W1P) Rabbit Monoclonal Antibody recognizes endogenous levels of total TRPV4 protein.

Species Reactivity:

Human, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro838 of human TRPV4 protein.

Background

TRPV4 is a member of the transient receptor potential vanilloid (TRPV) family of ion channels, and functions as a Ca²⁺-permeant non-selective cation channel. TRPV4 channels are expressed in many cell types, with particular abundance in sensory and spinal neurons (1). TRPV4 channels play a role in maintaining cellular homeostasis, by facilitating transmembrane Ca²⁺ transport in response to various stimuli, including thermal stress, fatty acid metabolites, and hypotonicity (2). Mutations in the TRPV4 gene have consequently been attributed to a variety of pathological conditions. For example, constitutively active TRPV4 mutants can lead to excess Ca²⁺ influx, resulting in toxicity and degeneration of peripheral nerves (3). TRPV4-dependent Ca²⁺ influx was also shown to mediate strain-induced and TGFβ1-induced epithelial-mesenchymal transition (EMT), suggesting a mechanistic role for TRPV4-mediated Ca²⁺ transport in fibrosis and oncogenesis (4). Consistent with this, studies in capillary endothelial cells showed that mechanical strain-induced Ca²⁺ influx through TRPV4 promote focal adhesion and stress fiber remodeling, mediated specifically through integrins, PI3K, and downstream kinases including Rho and ROCK (5).

Alternate Names

BCYM3; CMT2C; HMSN2C; Osm-9-like TRP channel 4; OSM9-like transient receptor potential channel 4; osmosensitive transient receptor potential channel 4; OTRPC4; SMAL; SPSMA; SSQTL1; Transient receptor potential cation channel subfamily V member 4; transient receptor potential cation channel, subfamily V, member 4; Transient receptor potential protein 12; TRP12; TRPV4; Vanilloid receptor-like channel 2; Vanilloid receptor-like protein 2; vanilloid receptor-related osmotically activated channel; Vanilloid receptor-related osmotically-activated channel; VR-OAC; VRL-2; VRL2; VROAC

Database Links

UniProt ID: Q9HBA0

Entrez-Gene Id: 59341

Limited Uses

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