|H||Endogenous||19, 22||Rabbit IgG|
Western blot analysis of recombinant human TSLP protein (hTSLP) using TSLP (D7U1X) Rabbit mAb.Learn more about how we get our images.
Western blot analysis of extracts from NHBE (normal human bronchial epithelial cells), untransfected (-) or transfected with poly(I:C) (10 μg/ml, 7 hr; +), using TSLP (D7U1X) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Brefeldin A #9972 was added to cells at 300 ng/ml one hour after the start of the transfection.Learn more about how we get our images.
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody datasheet or product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised November 2013
Reprobing of an existing membrane is a convenient means to immunoblot for multiple proteins independently when only a limited amount of sample is available. It should be noted that for the best possible results a fresh blot is always recommended. Reprobing can be a valuable method but with each reprobing of a blot there is potential for increased background signal. Additionally, it is recommended that you verify the removal of the first antibody complex prior to reprobing so that signal attributed to binding of the new antibody is not leftover signal from the first immunoblotting experiment. This can be done by re-exposing the blot to ECL reagents and making sure there is no signal prior to adding the next primary antibody.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalently purified water.
posted June 2005
revised October 2016
Protocol Id: 10
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
TSLP (D7U1X) Rabbit mAb recognizes endogenous levels of total TSLP protein. This antibody recognizes a 70 kDa protein of unknown identity.
Monoclonal antibody is produced by immunizing animals with recombinant human TSLP protein.
TSLP (thymic stromal lymphopoietin) is a cytokine produced by epithelial cells at barrier surfaces and granulocytes (1). TSLP acts on dendritic cells, lymphocytes, and granulocytes by binding to a heterodimer composed of TSLPR (TSLP receptor) and IL-7Rα (2-5). Constitutive expression of TSLP at barrier surfaces contributes to homeostasis by promoting generation of noninflammatory dendritic cells (6). TSLP promotes the differentiation of Th2 cells and type 2 immune responses both in response to helminth infection and in the context of atopic diseases including asthma and atopic dermatitis (7-13).
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. Tween is a registered trademark of ICI Americas, Inc.
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|97630S||100 µl (10 western blots)||$ 255.0|