Render Target: STATIC
Render Timestamp: 2024-11-06T10:58:17.688Z
Commit: 642d75590e907c0f7dfc7c6e3b846bcc0b02197c
XML generation date: 2024-09-30 01:58:41.145
Product last modified at: 2024-10-29T15:00:11.110Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

TUT4 (E9D9T) Rabbit mAb #15627

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 205
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    TUT4 (E9D9T) Rabbit mAb recognizes endogenous levels of total TUT4 protein. This antibody recognizes 82 kDa and 65 kDa bands of unknown origin.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His12 of human TUT4 protein.

    Background

    Terminal uridylyl transferase 4 and 7 (TUT4/7), also known as ZCCHC11 and ZCCHC6 respectively, are enzymes that posttranscriptionally uridylate mRNAs and miRNAs at the 3’ end (1,2). The TUT enzymes can monouridylate double-stranded RNA with a single nucleotide overhang, which in turn creates a recognition site for Dicer to process miRNAs (3). Many of the miRNAs recognized by TUT4/7 are involved in cell differentiation such as the HOX gene cluster (4). TUT4 and TUT7 have been shown to be recruited by Lin28 in stem cells to uridylate and block expression of let-7 miRNA, a microRNA with tumor suppressive qualities (3,5,6). TUT4 and TUT7 play a role in the mRNA decay pathway, where they polyuridylate targeted mRNAs to promote turnover (7). TUT4 and TUT7 have also been implicated in T cell activation, innate immune response, and antiviral defenses (8-10). Both TUT proteins have also been shown to prevent retrotransposition of LINE-1 elements, indicating a role in maintaining genomic stability (11).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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