Render Target: STATIC
Render Timestamp: 2024-12-12T11:03:17.793Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-11-22 17:16:06.522
Product last modified at: 2024-11-23T08:00:57.383Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

TWIST1 (E2M5V) Rabbit mAb #50850

Filter:
  • IHC

    Supporting Data

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Immunohistochemistry (Paraffin) 1:250 - 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    TWIST1 (E2M5V) Rabbit mAb recognizes endogenous levels of total TWIST1 protein. Non-specific cytoplasmic staining was observed in mouse brain by immunohistochemistry.

    Species Reactivity:

    Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly35 of human TWIST1 protein.

    Background

    TWIST1 is a basic helix-loop-helix (BHLH) transcription factor that functions as a master regulator of embryonic morphogenesis and plays essential roles in mesenchymal differentiation and osteogenic determination (1-3). Mutations affecting the BHLH domain of the TWIST1 gene have been associated with Saethre-Chotzen syndrome (SCS), an autosomal dominant craniosynostosis disorder causing craniofacial and limb abnormalities (4,5). TWIST1 is upregulated in various human tumors and may play a role in epithelial-mesenchymal transition (EMT) and metastasis (6,7). Upregulation of TWIST1 may contribute to resistance to taxol and microtubule-regulating drugs in tumors (8).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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