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9319
Tyrosinase (T311) Mouse mAb (IHC Specific)
Primary Antibodies
Monoclonal Antibody

Tyrosinase (T311) Mouse mAb (IHC Specific) #9319

Citations (4)
Filter:
  1. IHC
Immunohistochemistry Image 1: Tyrosinase (T311) Mouse mAb (IHC Specific)
Immunohistochemical analysis of human colon tissue using Tyrosinase (T311) Mouse mAb (IHC Specific). Note the lack of staining in the tyrosinase negative tissue.
Immunohistochemistry Image 2: Tyrosinase (T311) Mouse mAb (IHC Specific)
Immunohistochemical analysis of human melanoma tissue using Tyrosinase (T311) Mouse mAb (IHC Specific).

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 70-80
Source/Isotype Mouse IgG2a

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

Application Dilution
Immunohistochemistry (Paraffin) 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Tyrosinase (T311) Mouse mAb detects endogenous levels of total tyrosinase protein.

Species Reactivity:

Human

Source / Purification

Tyrosinase (T311) Mouse mAb is produced by immunizing animals with a purified recombinant tyrosinase.

Background

Tyrosinase, found in the membrane of melanosomes (1), is a key enzyme in the biosynthesis of melanin pigments (1,2). It is a melanocyte differentiation antigen and is expressed in normal melanocytes and malignant melanomas (1,3). Tyrosinase is implicated to be an antigen target for melanoma vaccines (1).
  1. Chen, Y.T. et al. (1995) Proc Natl Acad Sci USA 92, 8125-9.
  2. Kwon, B.S. (1993) J Invest Dermatol 100, 134S-140S.
  3. Kwon, B.S. et al. (1987) Proc Natl Acad Sci USA 84, 7473-7.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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