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R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

UBASH3A (F7T5P) Rabbit mAb #41606

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 74, 80
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:50 - 1:250
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    UBASH3A (F7T5P) Rabbit mAb recognizes endogenous levels of total UBASH3A protein. This antibody does not cross-react with UBASH3B protein and may detect bands of unknown origin around 25-30 kDa.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human UBASH3A protein.

    Background

    Ubiquitin-associated and SH3 domain-containing protein A (UBASH3A), also referred to as Suppressor of T cell receptor signaling 2 (STS-2), is an atypical protein tyrosine phosphatase that is primarily expressed in T cells (1). UBASH3A is a modular protein that contains an N-terminal UBA domain that can interact with ubiquitin, a central SH3 domain that mediates protein-protein interactions, and a C-terminal histidine phosphatase domain (1,2). Although it displays limited phosphatase activity compared to its homolog UBASH3B, UBASH3A functions as a negative regulator of T cell activation by downregulating the TCR-CD3 complex in a phosphatase-independent manner (3). UBASH3A also associates with the E3 ubiquitin ligase Cbl-b, which further dampens T cell activation through downregulation of CD28 (3). Due to its role in regulating T cell responses, UBASH3A dysfunction has been linked to several autoimmune disorders, including type 1 diabetes, rheumatoid arthritis, celiac disease, and systemic lupus erythematosus (4-7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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