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Render Timestamp: 2025-03-14T09:51:36.622Z
Commit: a619ae74f66dae0f27639e88da12bcf600e46428
XML generation date: 2025-03-07 13:10:48.630
Product last modified at: 2025-01-01T09:04:36.435Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. UBE1L/UBA7 (D6U4S) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

UBE1L/UBA7 (D6U4S) Rabbit mAb #61266

Filter:
  • WB

    Supporting Data

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 110
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    UBE1L/UBA7 (D6U4S) Rabbit mAb recognizes endogenous levels of total UBE1L/UBA7 protein. This antibody does not cross-react with either UBE1/UBA1 or UBE1L2/UBA6 proteins.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human UBE1L/UBA7 protein.

    Background

    Interferon-stimulated 15 kDa protein (ISG15), also known as ubiquitin cross-reactive protein (UCRP), is a member of the ubiquitin-like protein family and functions in various biological pathways from pregnancy to innate immune responses (1). Expression of ISG15 is stimulated by cellular exposure to type 1 interferons α and β, in addition to infection with viruses such as influenza B (2,3). After exposure to type I interferons, both lymphocytes and monocytes, in addition to some fibroblasts and epithelial cells, release ISG15 into culture medium (1,4). ISG15 has been shown to function as a cytokine, stimulating interferon γ secretion by monocytes and macrophages, proliferation of natural killer cells, and chemotactic responses in neutrophils (4,5). ISG15 has also been shown to function intracellularly, being covalently conjugated to other proteins by E1 (Ube1L), E2 (UbcH8) and E3 ligases via a multi-step process analogous to ubiquitination (6,7). ISG15 is removed from proteins by the ubiquitin processing protease Ubp43 (8). ISG15-protein conjugation (ISGylation) is induced by type 1 interferons, and target proteins include the serine protease inhibitor Serpin 2A, PLCγ1, ERK1/2, Jak1 and Stat1 (9,10). Unlike ubiquitination, ISGylation does not target proteins for degradation, rather ISGylation increases Jak1 and Stat1 activity, enhancing the cellular response to interferons (11).
    Ubiquitin-activating enzyme E1-like protein/Ubiquitin-activating enzyme 7 (UBE1L/UBA7) is the activating enzyme for ISG15. Research studies have suggested that loss of UBE1L/UBA7 expression contributes to the development of lung cancer due to compromised inhibition of cyclin D1 expression (12-15). UBE1L/UBA7 has also been implicated in the pathogenesis of acute promyelocytic leukemia through a mechanism in which UBE1L/UBA7 drives ISG15ylation of the oncogenic PML-RARα fusion protein to promote its degradation (16,17).
    1. Ritchie, K.J. and Zhang, D.E. (2004) Semin. Cell Dev. Biol. 15, 237-246.
    2. Korant, B.D. et al. (1984) J. Biol. Chem. 259, 14835-14839.
    3. Haas, A.L. et al. (1987) J. Biol. Chem. 262, 11315-11323.
    4. Knight, E. and Cordova, B. (1991) J. Immunol. 146, 2280-2284.
    5. D'Cunha, J. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 211-215.
    6. Loeb, K.R. and Haas, A.L. (1992) J. Biol. Chem. 267, 7806-7813.
    7. Zhao, C. et al. (2005) Proc. Natl. Acad. Sci. USA 102, 10200-10205.
    8. Malakhov, M.P. et al. (2002) J. Biol. Chem. 277, 9976-9981.
    9. Malakhov, M.P. et al. (2003) J. Biol. Chem. 278, 16608-16613.
    10. Hamerman, J.A. et al. (2002) J. Immunol. 168, 2415-2423.
    11. Malakhova, O.A. et al. (2003) Genes Dev. 17, 455-460.
    12. McLaughlin, P.M. et al. (2000) Int J Cancer 85, 871-6.
    13. Kok, K. et al. (1995) Gene Expr 4, 163-75.
    14. Pitha-Rowe, I. et al. (2004) Cancer Res 64, 8109-15.
    15. Feng, Q. et al. (2008) Mol Cancer Ther 7, 3780-8.
    16. Shah, S.J. et al. (2008) Mol Cancer Ther 7, 905-14.
    17. Kitareewan, S. et al. (2002) Proc Natl Acad Sci U S A 99, 3806-11.

    Database Links

    UniProt ID: P41226


    Entrez-Gene Id: 7318


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    For Research Use Only. Not For Use In Diagnostic Procedures.
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