Render Target: STATIC
Render Timestamp: 2024-11-08T10:56:17.843Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-10-29 22:01:06.991
Product last modified at: 2024-11-04T09:00:11.252Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

UBF (F2F1O) Rabbit mAb #35663

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 90
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    UBF (F2F1O) Rabbit mAb recognizes endogenous levels of total UBF protein, including both isoforms UBF1 and UBF2.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human UBF protein.

    Background

    RNA polymerase I (RNAPI) is a large multi-protein complex that functions as a DNA-dependent, RNA polymerase, which is primarily responsible for the transcription of ribosomal RNA (rRNA) genes. The largest subunit, Rpa194, or POLR1A, along with selectivity factor 1 (SL1) and the transactivator protein upstream binding factor (UBF), makes up the core transcriptional machinery of the RNAPI complex (1-3). UBF is a nucleolar protein that functions as a dual transcription factor and epigenetic remodeler to promote rRNA transcription (4). ChIP-seq studies show that UBF binds ribosomal DNA (rDNA) at the promoter sequence and throughout the transcribed regions (4,5). At the promoter region, UBF associates with SL1 to drive formation of the preinitiation complex (PIC) (6,7). UBF also promotes rRNA transcription by displacing linker histone H1 and keeping rDNA in an active euchromatin state (8,9). Due to its critical role in ribosome biogenesis, defects in UBF have been linked to several ribosomopathies and neurological disorders (10). Specifically, a gain-of-function E210K mutation in UBF has been linked to multiple cases of pediatric neurodegeneration (11).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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