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300 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

UBR5 Antibody recognizes endogenous levels of total UBR5 protein.

Species predicted to react based on 100% sequence homology: Dog, Pig

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human UBR5 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Human E3 identified by differential display (UBR5/EDD) is a HECT domain-containing ubiquitin E3 ligase of the N-end rule pathway that promotes the ubiquitination and proteasomal degradation of proteins harboring N-degrons (1-3). UBR5 represents an ortholog of HYD, the Drosophila hyperplastic discs tumor suppressor gene product but has been found to be overexpressed in breast and ovarian cancers, suggesting a possible role in promoting tumor development (4,5). Research studies have demonstrated that UBR5 is functional within the nucleus as it participates in DNA damage signaling by controlling the activities of Chk2, TopBP1, and RNF168 (6-9). Recently, UBR5 was shown to play a novel role in immune cell function by regulating RORγt stability and IL-17 production by Th17 cells (10).

1.  Sriram, S.M. et al. (2011) Nat Rev Mol Cell Biol 12, 735-47.

2.  Callaghan, M.J. et al. (1998) Oncogene 17, 3479-91.

3.  Tasaki, T. et al. (2005) Mol Cell Biol 25, 7120-36.

4.  Clancy, J.L. et al. (2003) Oncogene 22, 5070-81.

5.  O'Brien, P.M. et al. (2008) Br J Cancer 98, 1085-93.

6.  Munoz, M.A. et al. (2007) Cell Cycle 6, 3070-7.

7.  Henderson, M.J. et al. (2006) J Biol Chem 281, 39990-40000.

8.  Honda, Y. et al. (2002) J Biol Chem 277, 3599-605.

9.  Gudjonsson, T. et al. (2012) Cell 150, 697-709.

10.  Rutz, S. et al. (2015) Nature 518, 417-21.

Entrez-Gene Id 51366
Swiss-Prot Acc. O95071

For Research Use Only. Not For Use In Diagnostic Procedures.
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UBR5 Antibody