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VAMP3 (D9S6K) Rabbit Monoclonal Antibody #61451

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  • WB
  • IHC

    Product Specifications

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 13
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:800

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    VAMP3 (D9S6K) Rabbit Monoclonal Antibody recognizes endogenous levels of total VAMP3 protein.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala8 of human VAMP3 protein.

    Background

    Proteins in the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex are integral membrane proteins involved in vesicle transport and membrane fusion by pairing of vesicular SNAREs (v-SNAREs) with cognate target SNAREs (t-SNAREs) (reviewed in 1,2). Vesicle-associated membrane protein 3 (VAMP3), also known as cellubrevin, has a broad tissue distribution and localizes to endosomal compartments (3). VAMP3 interacts with the t-SNAREs syntaxin1, syntaxin4, SNAP23, and SNAP25 (4,5). Research studies indicate that VAMP3 is involved in transferrin receptor recycling to the plasma membrane (6) and T cell receptor recycling to immunological synapses (7). Inhibition of VAMP3 with tetanus toxin impairs membrane trafficking during cell migration (8).

    Alternate Names

    CEB; Cellubrevin; SYB3; Synaptobrevin-3; VAMP-3; VAMP3; vesicle associated membrane protein 3; Vesicle-associated membrane protein 3; vesicle-associated membrane protein 3 (cellubrevin)

    For Research Use Only. Not for Use in Diagnostic Procedures.
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