VCre Recombinase (F3Q3G) Rabbit mAb #88113
- WB
- IP
- IF
Supporting Data
| REACTIVITY | All |
| SENSITIVITY | Transfected Only |
| MW (kDa) | 42 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- All-All Species Expected
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:1600 - 1:3200 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Similar to Cre/Lox, two additional site-specific recombination systems have been identified: VCre/VloxP and SCre/SloxP. Although the structure of their recognition sites are identical, Cre, VCre, and SCre recombinases do not cross-react with each other, allowing for in tandem use (7).
- Abremski, K. et al. (1983) Cell 32, 1301-11.
- Sternberg, N. et al. (1981) Cold Spring Harb Symp Quant Biol 45 Pt 1, 297-309.
- Abremski, K. and Hoess, R. (1984) J Biol Chem 259, 1509-14.
- Qin, M. et al. (1994) Proc Natl Acad Sci U S A 91, 1706-10.
- Lakso, M. et al. (1992) Proc Natl Acad Sci U S A 89, 6232-6.
- Guo, F. et al. (1997) Nature 389, 40-6.
- Suzuki, E. and Nakayama, M. (2011) Nucleic Acids Res 39, e49.
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