|SignalStain® Vibrant Red Diluent||120|
|SignalStain® Vibrant Red Reagent 1||2.5|
|SignalStain® Vibrant Red Reagent 2||2.5|
1. To prepare the Vibrant Red working solution, to 5 ml SignalStain® Vibrant Red Diluent add 2 drops SignalStain® Vibrant Red Reagent 1 and 2 drops SignalStain® Vibrant Red Reagent 2. Mix well before use.
2. After incubation with SignalStain® Boost IHC Detection Reagent or other AP-based detection systems, wash slides and incubate with the working solution at room temperature for 20-30 minutes.
3. Wash slides in TBS-T for 5 minutes and then rinse slides in water. Counterstain, if desired.
Vibrant Red is compatible with permanent and aqueous mounting. Please note, permanent mounting requires dehydration through ethanol and clearing in xylene.
Optimal development times should be determined by the investigator. Working solutions should be used within 15 minutes of preparation. Do not heat Vibrant Red kit components or working solution.
The reaction product is also fluorescent and non-fading and can be viewed with TRITC filter systems.
Appropriate personal protective equipment should be worn when handling Vibrant Red. Dispose of in accordance with local regulations.
Substrate kit should be stored at 4ºC, protected from light. Each kit is stable for 12 months upon receipt if the reagents are not combined.
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