Interested in promotions? | Click here >>
40211
Vinculin (E1E9V) XP® Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

Vinculin (E1E9V) XP® Rabbit mAb (BSA and Azide Free) #40211

Reviews ()
Citations (0)
Filter:
  1. WB
  2. IHC
Western Blotting Image 1: Vinculin (E1E9V) XP® Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from various cell lines using Vinculin (E1E9V) XP® Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb, #6487 (lower). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: Vinculin (E1E9V) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using Vinculin (E1E9V) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: Vinculin (E1E9V) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human breast ductal carcinoma in situ using Vinculin (E1E9V) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: Vinculin (E1E9V) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded mouse spleen using Vinculin (E1E9V) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: Vinculin (E1E9V) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded Hs578T (left) and Jurkat (right) cell pellets using Vinculin (E1E9V) XP(R) Rabbit mAb. Data were generated using the standard formulation of this product.
To Purchase # 40211
Cat. # Size Qty. Price
40211SF
100 µg $ 746

Supporting Data

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 124
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #13901. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free. For standard formulation of this product see product #13901

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

Vinculin (E1E9V) XP® (BSA and Azide Free) recognizes endogenous levels of total vinculin protein. This antibody also reacts with metavinculin, a 145 kDa splice variant of vinculin.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human vinculin protein.

Background

Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1-4). Three structural vinculin domains include an amino-terminal head, a short, flexible proline-rich region and a carboxy-terminal tail (1). In the inactive state, the head and tail domains of vinculin interact to form a closed confirmation. The open and active form of vinculin translocates to focal adhesions where it is thought to be involved in anchoring F-actin to the membrane and regulation of cell migration (2). Phospholipid binding to the tail domain and subsequent phosphorylation of vinculin at Ser1033 and Ser1045 by PKC-α and Tyr100 and Tyr1065 by Src kinases weakens the head-tail interaction (5,6). This change in vinculin allows the binding of a number of other proteins, including talin, α-actinin and paxillin, which disrupts the head-tail interaction and initiates the conformational change from the inactive to active state (2,4). Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (7,8). This is supported by a demonstrated relationship between decreased vinculin expression and increased carcinogenesis and metastasis in colorectal carcinoma (9).
  1. Izard, T. et al. (2004) Nature 427, 171-5.
  2. Humphries, J.D. et al. (2007) J Cell Biol 179, 1043-57.
  3. Witt, S. et al. (2004) J Biol Chem 279, 31533-43.
  4. Xu, W. et al. (1998) Development 125, 327-37.
  5. Ziegler, W.H. et al. (2002) J Biol Chem 277, 7396-404.
  6. Zhang, Z. et al. (2004) Mol Biol Cell 15, 4234-47.
  7. Rodríguez Fernández, J.L. et al. (1993) J Cell Biol 122, 1285-94.
  8. Samuels, M. et al. (1993) J Cell Biol 121, 909-21.
  9. Yang, H.J. et al. (2010) Cancer Invest 28, 127-34.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.