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96632
SignalStain® Vivid Purple Peroxidase Substrate Kit
Primary Antibodies
Detection System Kit

SignalStain® Vivid Purple Peroxidase Substrate Kit #96632

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Immunohistochemistry Image 1: SignalStain® Vivid Purple Peroxidase Substrate Kit

Immunohistochemical analysis of paraffin embedded human breast carcinoma using E-Cadherin (4A2) Mouse mAb #14472 (purple) and Vimentin (D21H3) XP® Rabbit mAb #5741 (brown). Note: SignalStain® Boost IHC Detection Reagent (HRP, Mouse) #8125 and SignalStain® Vivid Purple Peroxidase Substrate Kit were used to detect E-Cadherin (4A2) Mouse mAb. SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) #8114 and SignalStain® DAB Substrate Kit #8059 were used to detect Vimentin (D21H3) XP® Rabbit mAb.

Immunohistochemistry Image 2: SignalStain® Vivid Purple Peroxidase Substrate Kit

Immunohistochemical analysis of paraffin-embedded human melanoma using Sox10 (E6B6I) XP® Rabbit mAb #69661.

Immunohistochemistry Image 3: SignalStain® Vivid Purple Peroxidase Substrate Kit

Immunohistochemical analysis of paraffin-embedded human melanoma using MLANA/MART-1 (E9Q4O) XP® Rabbit mAb #64718.

To Purchase # 96632S
Product # Size Price
96632S
1 Kit  (1200 slides) $ 215

Product Usage Information

1. To prepare the Vivid Purple working solution, to 5 ml SignalStain® Vivid Purple Diluent add 3 drops SignalStain® Vivid Purple Reagent 1, 3 drops SignalStain® Vivid Purple Reagent 2, 3 drops SignalStain® Vivid Purple Reagent 3, and 3 drops SignalStain® Vivid Purple Reagent 4. Mix well before use.

2. After incubation with SignalStain® Boost IHC Detection Reagent or other HRP-based detection systems, wash slides and incubate with the working solution at room temperature for 2-15 minutes. Optimal development time should be determined by the investigator.

3. Wash slides for 5 minutes in water.

4. Counterstain, if desired.

5. Dehydrate, clear and permanently mount in a non-aqueous mounting medium. Do not use recycled alcohols with this product.

Prolonged incubation in alcohol may decrease sensitivity.

Unused working solution is stable up to 14 days if stored at 2-8ºC. Working solution may change color during this time with no impact on quality or intensity of the staining. If a precipitate forms, filter through a 0.45 μm filter.

Appropriate personal protective equipment should be worn when handling Vivid Purple. Dispose of in accordance with local regulations.

Storage

Substrate kit should be stored at 4ºC, protected from light. Each kit is stable for 12 months upon receipt if the reagents are not combined.

Product Description

The SignalStain® Vivid Purple Peroxidase Substrate Kit contains all of the necessary reagents to prepare a working solution of Vivid Purple for staining tissue sections. The Vivid Purple working solution reacts with peroxidase (HRP) detection systems such as the SignalStain® Boost IHC Detection Reagents (HRP, Rabbit #8114, and HRP, Mouse #8125), yielding a purple reaction product that can provide color contrast in pigmented tissues such as melanoma.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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SignalStain is a trademark of Cell Signaling Technology, Inc.
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