Render Target: STATIC
Render Timestamp: 2024-10-11T09:54:42.134Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-09-30 01:58:06.422
Product last modified at: 2024-09-30T08:00:18.631Z
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PDP - Template Name: Monoclonal Antibody (Epitope Tag)
PDP - Template ID: *******f943b5c
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

VSV-G Tag (E8S5G) Rabbit mAb #93372

Filter:
  • WB
  • IP
  • IF

    Supporting Data

    REACTIVITY All
    SENSITIVITY Transfected Only
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • All-All Species Expected 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Immunofluorescence (Immunocytochemistry) 1:100 - 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    VSV-G Tag (E8S5G) Rabbit mAb detects recombinant proteins containing the VSV-G epitope tag. This antibody recognizes the VSV-G tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. VSV-G Tag (E8S5G) Rabbit mAb detects exogenously expressed VSV-G tagged proteins in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated.

    Species Reactivity:

    All Species Expected

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing the vesicular stomatitis virus glycoprotein epitope (YTDIEMNRLGK).

    Background

    Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

    The VSV-G tag is derived from an epitope from the C-terminal region of vesicular stomatitis virus glycoprotein (1). VSV-G is widely used both as an epitope tag in expression vectors and for pseudotyping lentiviral vectors due to the stability and broad host range of the resulting pseudotypes (2,3).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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