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PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools


Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Wnt3a (C64F2) Rabbit mAb 2721 20 µl
Western Blotting Immunoprecipitation
M 42 Rabbit IgG
Wnt5a/b (C27E8) Rabbit mAb 2530 20 µl
Western Blotting
H 45 Rabbit IgG
Phospho-LRP6 (Ser1490) Antibody 2568 20 µl
Western Blotting Immunoprecipitation
H 180, 210 Rabbit 
Dvl2 (30D2) Rabbit mAb 3224 20 µl
Western Blotting Immunoprecipitation
H M R Mk 90 to 95 Rabbit 
LRP6 (C47E12) Rabbit mAb 3395 20 µl
Western Blotting Immunoprecipitation
H M 180, 210 Rabbit IgG
Dvl3 Antibody 3218 20 µl
Western Blotting Immunoprecipitation
H M R Hm Mk Mi B 88 to 93 Rabbit 
Naked1 (C30F10) Rabbit mAb 2201 20 µl
Western Blotting Immunoprecipitation
H 59, 61 Rabbit IgG
Naked2 (C67C4) Rabbit mAb 2073 20 µl
Western Blotting Immunoprecipitation
H 59, 61 Rabbit 
Axin1 (C76H11) Rabbit mAb 2087 20 µl
Western Blotting Immunoprecipitation Immunohistochemistry
H M 110 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting

Product Description

The Wnt Signaling Antibody Sampler Kit provides an economical means of detecting integral proteins within the Wnt signaling pathway. The kit contains enough primary and secondary antibody to perform two Western blots with each.

Specificity / Sensitivity

Wnt3a (C64F2) Rabbit mAb detects transfected Wnt3a protein in L cells and the conditioned medium from L/Wnt3a cells. Phospho-LRP6 (Ser1490) Antibody detects endogenous levels of LRP6 protein when phosphorylated at Ser1490. The remaining antibodies in the Wnt Signaling Sampler Kit detect endogenous levels of their respective total proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu256 of human Wnt3a, Glu176 of human Wnt5a, Met988 of human LRP6, Gly176 of human Dvl2, Leu53 of human Naked1, and Ala217 of human Naked2. The monoclonal antibody for Axin1 is produced by immunizing animals with recombinant human Axin1. The phospho-specific polyclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser1490 of human LRP6. The total polyclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the carboxy terminus of human Dvl3. Polyclonal antibodies are purified by Protein A and peptide affinity chromotagraphy.

The Wnt family includes several secreted glycoproteins that play important roles in animal development (1). Aberrant activation of Wnt signaling pathways is involved in several types of cancers (2). Wnt members bind to the Frizzled family of proteins and activate several signaling pathways (3). The canonical Wnt/β-catenin pathway requires a coreceptor from proteins belonging to the low-density lipoprotein receptor (LDLR) family, such as LRP5 and LRP6 (4). Upon stimulation with Wnt, LRP6 is phosphorylated by kinases such as GSK-3 and CK1 and subsequently recruits Axin to the membrane (5-7). In the absence of Wnt stimulation, the multidomain scaffold proteins Axin1 and Axin2 negatively regulate Wnt signaling. Axin1 complexes with APC, GSK-3β, Dvl and β-catenin and promotes the GSK-3β-mediated phosphorylation and subsequent degradation of β-catenin (8-9). The Disheveled (Dsh) proteins (Dvl1, Dvl2 and Dvl3) inhibit glycogen synthase kinase-3β, thereby promoting β-catenin stabilization (4,10). Naked inhibits the canonical Wnt/β-catenin pathway by binding to Dsh proteins and directing Dsh activity towards the planar cell polarity pathway (11-12).

1.  Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305.

2.  Luo, W. and Lin, S.C. Neurosignals 13, 99-113.

3.  Salahshor, S. and Woodgett, J.R. (2005) J. Clin. Pathol. 58, 225-236.

4.  Boutros, M. and Mlodzik, M. (1999) Mech. Dev. 83, 27-37.

5.  Wharton, K.A. et al. (2001) Dev. Biol. 234, 93-106.

6.  Tamai, K. et al. (2000) Nature 407, 530-535.

7.  Yan, D. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 3802-3807.

8.  Giles, R.H. et al. (2003) Biochim. Biophys. Acta. 1653, 1-24.

9.  Logan, C.Y. and Nusse, R. (2004) Annu Rev Cell Dev Biol 20, 781-810.

10.  Kohn, A.D. and Moon, R.T. Cell Calcium 38, 439-46.

11.  Zeng, X. et al. (2005) Nature 438, 873-7.

12.  Davidson, G. et al. (2005) Nature 438, 867-72.

Entrez-Gene Id 8312 , 1856 , 1857 , 4040 , 85407 , 85409 , 89780 , 7474 , 81029
Swiss-Prot Acc. O15169 , O14641 , Q92997 , O75581 , Q969G9 , Q969F2 , P56704 , P41221 , Q9H1J7

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.

Wnt Signaling Antibody Sampler Kit