Render Target: STATIC
Render Timestamp: 2024-11-01T10:04:37.839Z
Commit: 23cb9f61fe67e1e9093fd644a533c4ff516a6463
XML generation date: 2024-09-30 01:59:05.667
Product last modified at: 2024-09-30T08:01:24.995Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

WWP2 (E8W8A) Rabbit mAb #41182

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 110
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    WWP2 (E8W8A) Rabbit mAb recognizes endogenous levels of total WWP2 protein.

    Species Reactivity:

    Human

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val349 of human WWP2 protein.

    Background

    Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Ubiquitin is first activated by forming a thiolester complex with the activation component E1. The activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, and then from E2 to ubiquitin ligase E3 for final delivery to the ε-NH2 of the target protein lysine residue (1-3). Research studies suggest that activated E2 associates transiently with E3, and the dissociation is a critical step for ubiquitination (4).

    WWP2 (WW domain-containing protein 2, AIP2) is a member of the NEDD4 family of E3 ligases, which play an important role in protein ubiquitination. WWP2 may play a role in multiple processes, including chondrogenesis and oncogenic signaling pathway regulation via interactions with Smad proteins and the tumor suppressor PTEN. It has recently become clear that aberrant activity of WWP2 may play a role in multiple tumor types and promotes tumorigenesis through the PTEN/Akt signaling pathway. Recently, accumulating evidence indicates that WWP2 plays a crucial part in the pathogenesis of different tumor types (5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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