Render Target: STATIC
Render Timestamp: 2024-11-08T09:46:01.073Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-07-24 19:01:09.219
Product last modified at: 2024-10-01T16:45:09.717Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

XRCC4 Antibody #23908

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 50
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    XRCC4 Antibody recognizes endogenous levels of total XRCC4 protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro268 of human XRCC4 protein. Antibodies are purified by peptide affinity chromatography.

    Background

    DNA double-strand breaks (DSBs) are potentially hazardous lesions that can be induced by ionizing radiation (IR), radiomimetic chemicals, or DNA replication inhibitors. Cells recognize and repair DSBs via two distinct but partly overlapping signaling pathways: non-homologous end joining (NHEJ) and homologous recombination (HR). DNA repair via the HR pathway is restricted to S and G2 phases of the cell cycle, while NHEJ can occur during any phase. NHEJ machinery is also utilized in V(D)J recombination, a process that generates diversity in immunoglobulin and T cell receptor genes. Defects in both pathways have been associated with human disease, including cancer (1).

    DNA repair through the NHEJ pathway involves a core group of proteins that includes the Ku heterodimer (Ku70/Ku80), DNA-PKcs, DNA ligase IV, X-ray repair cross-complementing protein 4 (XRCC4), XLF, and PAXX (Paralog of XRCC4 and XLF, also known as C9orf142 or XLS). XRCC4 interacts with XLF and promotes the ligation of DNA strands by DNA ligase IV (2).

    Mutations and polymorphisms in XRCC4 have been linked to human disease, including microcephaly, dwarfism, and cancer susceptibility (3-5). Knockdown of XRCC4 expression in hepatocellular carcinoma (HCC) cells and triple-negative breast cancer cells increases sensitivity to doxorubicin and ionizing radiation, respectively (6,7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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